Effect of Acrostalagmus luteoalbus ACRO1 on Mortality of the Second-stage Juveniles of the Citrus Nematode Tylenchulus semipenetrans under Laboratory Conditions

Document Type : Research Paper

Authors

Department of Plant Protection, School of Agriculture, Shiraz University, Shiraz, I. R. Iran

10.22099/iar.2025.51197.1634

Abstract

This study investigates the nematicidal activity of Acrostalagmus luteoalbus ACRO1 as a potential biocontrol agent against the citrus nematode Tylenchulus semipenetrans under laboratory conditions. The ACRO1 isolate was cultured in potato extract-dextrose broth, and its nematicidal metabolites were extracted using ethyl acetate. Its effectiveness was assessed in comparison to Purpureocillium lilacinum, a known biocontrol agent, and abamectin, a chemical nematicide. ACRO1 demonstrated remarkable nematicidal efficacy, achieving an average mortality rate of 81.5% within 72 hours, significantly surpassing P. lilacinum (46.3%) and the negative control (6.5%). In a secondary experiment, ACRO1 induced 58.7% mortality in 72 hours, again outperforming P. lilacinum (26.2%) and the control (6.25%). Its cell-free culture extract exhibited rapid activity, reaching 76.0% mortality within 24 hours, while its metabolites achieved complete nematicidal efficacy with 100% mortality. By comparison, abamectin induced mortality rates of 53.2%, 62.2%, and 100% at concentrations of 5 ppm, 10 ppm, and 3%, respectively. These findings underscore ACRO1’s potential as an effective and environmentally friendly alternative to synthetic nematicides, offering a sustainable solution for managing the citrus nematode, a persistent threat to global citrus production.

Keywords

Main Subjects

Article Title [Persian]

تأثیر Acrostalagmus luteoalbus ACRO1 بر مرگ و میر سن دو نماتد مرکبات Tylenchulus semipenetrans در شرایط آزمایشگاهی

Authors [Persian]

  • رضا قرین
  • اکبر کارگربیده
  • مریم میرطالبی
بخش گیاهپزشکی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ج. ا. ایران
Abstract [Persian]

در این مطالعه، توانایی نماتدکشی Acrostalagmus luteoalbus ACRO1 در برابر نماتد مرکبات Tylenchulus semipenetrans به‌عنوان یک عامل مهار زیستی بالقوه در شرایط آزمایشگاهی مورد بررسی قرار گرفت. جدایه ACRO1 در محیط کشت مایع عصاره سیب‌زمینی-دکستروز کشت داده شد و متابولیت‌های آن با استفاده از اتیل استات استخراج شدند. کارایی جدایه ACRO1 در برابر نماتد مرکبات با عامل مهارزیستی شناخته شده Purpureocillium lilacinum و نماتدکش شیمیایی آبامکتین مقایسه گردید. نتایج آزمایش نشان داد که 72 ساعت پس از شروع آزمایش جدایه ACRO1 باعث میانگین مرگ و میر 81/5% شد که بسیار بالاتر از شاهد منفی (6/5%) و P. lilacinum (46/3%) بود. در آزمایش دوم جدایهACRO1 به طور متوسط نرخ مرگ و میر 58/7% را در 72 ساعت پس از شروع آزمایش نشان داد که بسیار بالاتر از شاهد منفی (6/25%) و P. lilacinum (26/2%) بود. عصاره آنزیمی بدون اسپور جدایه ACRO1 منجر به نرخ مرگ و میر 76% پس از 24 ساعت شد در حالی که نرخ مرگ و میر متابولیت‏های ثانویه این جدایه 100% بود. در مقایسه، آبامکتین شیمیایی میزان مرگ و میر به ترتیب 53/2، 62/2 و 100 درصد در غلظت های 5 پی پی ام، 10 پی پی ام و 3 درصد داشت. این یافته‌ها قابلیت جدایه ACRO1 را به‌عنوان یک جایگزین مؤثر و سازگار با محیط‌زیست برای نماتدکش‌های شیمیایی نشان می‌دهد، که می‌تواند برای مدیریت نماتد مرکبات به عنوان یک تهدید دائمی تولید جهانی مرکبات، رویکردی پایدار را ارائه دهد.

Keywords [Persian]

  • خصوصیت نماتدکشی
  • متابولیت‌های ثانویه قارچی
  • مهار زیستی

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Iran Agricultural Research
Volume 43, Issue 2
December 2024
Pages 121-126

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