Effects of aqueous extracts and total protein content from different plant species on the inhibition of Tobacco mosaic virus infection

Document Type : Research Paper

Authors

1 Department of Plant Protection, College of Agriculture, Shahrekord University, Shahrekord, I. R. Iran

2 Department of Horticultural Sciences, College of Agriculture, Shahrekord University, Shahrekord, I. R. Iran

10.22099/iar.2025.51800.1652

Abstract

This study examined the inhibitory properties of aqueous extract and total protein of four plant species: carnation (Dianthus caryophyllus), Indian privet (Clerodendrum inerme), prickly pear cactus (Opuntia stricta), and periwinkle (Catharanthus roseus) against Tobacco mosaic virus (TMV). The aqueous extract and total protein from carnation, Indian privet, and cactus significantly reduced TMV infection in the local lesion host, Nicotiana glutinosa. Conversely, the periwinkle extract did not demonstrate a significant inhibitory effect on TMV. Results showed that the inhibitory effects of carnation and Indian privet were more effective than those of the cactus extract. The inhibitory effects of the plant extracts applied on the N. glutinosa leaves diminished after washing with water. Bioassay tests using total protein from carnation, Indian privet, and cactus indicated that the inhibitory effects of these proteins were comparable to those of their respective aqueous extracts. Thus, it can be inferred that the inhibitory agents present in these plants are proteins. The findings from both the aqueous extracts and the proteins extracted from carnation, Indian privet, and cactus suggest that the inhibitory effects of these substances are localized and not systemic, being confined to the leaves that were treated either simultaneously or prior to the virus inoculation. When N. tabacum (var. Turkish), a systemic host, was inoculated with TMV mixed with these plant extracts, there was no evidence of inhibition or a reduction in the incubation period. Therefore, using these extracts to manage TMV infections in its main systemic hosts may be ineffective. 

Keywords

Main Subjects

Article Title [Persian]

اثر عصاره آبی و پروتئینهای چهار گونه گیاهی در بازداری از آلودگی ویروس موزاییک توتون

Authors [Persian]

  • فاطمه کاهانی 1
  • مجید صیام پور 1
  • مسعود قاسمی قهساره 2
1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه شهرکرد، شهرکرد، ج. ا. ایران
2 گروه باغبانی، دانشکده کشاورزی، دانشگاه شهرکرد، شهرکرد، ج. ا. ایران
Abstract [Persian]

در این تحقیق اثر بازدارندگی عصاره آبی و پروتئین‌های کل چهار گیاه شامل میخک (Dianthus caryophyllus)، شمشاد اهوازی (Clerodendrum inerme)، کاکتوس اپونتیا (Opuntia stricta) و پروانش (Catharanthus roseus) بر ویروس موزائیک توتون بررسی شد. عصاره و پروتئین‌های کل استخراج شده از میخک، شمشاد اهوازی و کاکتوس توانستند وقوع آلودگی‌های موفق ناشی از TMV را در میزبان لکه موضعی توتون گلوتینوزا (Nicotiana glutinosa) کاهش دهند. عصاره آبی پروانش تاثیر خاصی بر TMV نداشت. مقایسه اثر عصاره‌ها نشان داد که توان بازدارندگی عصاره میخک و شمشاد اهوازی با یکدیگر قابل مقایسه بوده و بیشتر از اثر بازدارندگی کاکتوس است. نتایج همچنین نشان داد که عامل بازدارنده در عصاره‌های میخک، شمشاد اهوازی و کاکتوس در سطح گیاهان تیمار شده فعالیت داشته و پس از شستشو با آب فعالیت آن از بین می‌رود. اثر بازدارندگی با استفاده از پروتئین کل استخراج شده از میخک، شمشاد اهوازی و کاکتوس مشابه با اثر عصاره‌های آبی این گیاهان بود. از این رو به‌احتمال عامل بازدارنده موجود در این گیاهان ماهیت پروتئینی دارد. نتایج به‌دست آمده نشان داد که اثر بازدارندگی عصاره‌های آبی یا پروتنین‌های میخک، شمشاد اهوازی سیتمیک نبوده و تنها در برگ‌هایی که قبل یا همزمان با تلقیح TMV مورد استفاده قرار گرفته بودند موثر بود. تلقیح همزمان عصاره گیاهان مذکور و TMV به میزبان سیستمیک توتون رقم تورکیش (N. tabacum var. Turkish) باعث هیچ گونه کنترل و یا کاهش دوره نهفتگی نشد. در نتیجه استفاده از این عصاره‌ها در کنترل TMV در میزبان‌های اصلی مانند توتون رقم تورکیش کارایی چندانی نشان نداد.

Keywords [Persian]

  • اثر ضدویروسی
  • سیستمیک
  • عصاره گیاهی
  • گلوتینوزا
  • لکه موضعی

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Iran Agricultural Research
Volume 43, Issue 2
December 2024
Pages 87-93

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