Investigation of the expression pattern of some nitrogen transporter genes in root in two different cultivars of wheat

Document Type : Research Paper

Authors

Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I. R. Iran

Abstract

In crop production, one particularly limiting factor is the availability of nitrogen (N) and N derivatives, as they are of much significance in plant metabolism and growth. Various nitrate transporter genes mediate the uptake, transport, and remobilization of N in the plants. However, their role is not quite understood with respect to grain filling stages and plant’s overall yield. Herein, the expression of a number of genes and enzymes with roles in N uptake and metabolism was measured over the course of anthesis and under N-treatment and N-deficit condition in two wheat genotypes, namely Morvarid and Gonbad. The samples were taken 10, 15, and 20 days after anthesis, which is a critical stage in grain filling, and the relation between gene expression, enzyme activity, and days after anthesis was demonstrated. It was observed that N-related gene expression significantly increases under N-deficit condition and over the course of anthesis, suggesting the significance of the N-related genes and enzyme in maintaining the metabolism and growth of the plants under N-deficit conditions.

Keywords

Article Title [Persian]

بررسی الگوی بیان برخی از ژن های ناقل نیتروژن در ریشه در دو رقم مختلف گندم

Authors [Persian]

  • سعید نواب پور
  • حوریه نجفی
  • فاطمه صحرایی قمش
گروه اصلاح نباتات و بیوتکنولوژی، دانشکده تولیدات گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ج. ا. ایران
Abstract [Persian]

در تولید محصولات زراعی، یکی از عوامل محدود کننده به ویژه در دسترس بودن نیتروژن (N) و مشتقات N است، زیرا آنها در متابولیسم و رشد گیاه اهمیت زیادی دارند. ژن های مختلف ناقل نیترات جذب، انتقال و جابجایی مجدد نیتروژن را در گیاهان واسطه می کنند. با این حال، نقش آنها با توجه به مراحل پر شدن دانه و عملکرد کلی گیاه کاملاً درک نشده است. در اینجا، بیان تعدادی از ژن‌ها و آنزیم‌های دارای نقش در جذب و متابولیسم نیتروژن در طول دوره گرده‌افشانی و در شرایط تیمار N و کمبود N در دو ژنوتیپ گندم مروارید و گنبد اندازه‌گیری شد. نمونه‌ها 10، 15 و 20 روز پس از گرده‌افشانی که مرحله‌ای حیاتی در پر شدن دانه است، گرفته شد و ارتباط بین بیان ژن، فعالیت آنزیم و روزهای پس از گرده‌افشانی نشان داده شد. مشاهده شد که بیان ژن مربوط به N به طور قابل توجهی در شرایط کمبود N و در طول دوره گرده‌افشانی افزایش می‌یابد، که نشان‌دهنده اهمیت ژن‌ها و آنزیم مربوط به N در حفظ متابولیسم و رشد گیاهان در شرایط کمبود N است.

Keywords [Persian]

  • بیان ژن ریشه
  • گندم
  • نیتروژن

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Iran Agricultural Research
Volume 42, Issue 1
June 2023
Pages 29-40

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