Quantitative expression analyses of Dipeptidyl peptidase and Cytochrome P450 Monooxygenase genes of Beauveria bassiana during growth on insect cuticles

Document Type : Research Paper

Authors

Faculty of Life Sciences & Biotechnology, Shahid Beheshti University, Tehran, I. R. Iran

Abstract

Beauveria bassiana is an entomopathogenic fungus that acts as a biological control agent and attacks varieties of insects by degrading and penetrating their cuticles. The relevant molecular basis of this action was investigated by comparing the expression analyses of dipeptidyl peptidase and cytochrome P450 monooxygenase, candidate genes involved in fungal pathogenesis, via Real-Time PCR. B. bassiana was grown in an artificial medium and in the cuticle extract media isolated from four species belonging to different insect orders. The expression of the two genes in the fungus was examined in different culture media. The results showed that dipeptidyl peptidase was upregulated in media containing insect cuticular extracts compared to the control synthetic medium, while Cytochrome P450 monooxygenase showed reduced expression in abundance. The effect of cuticle types in inducing gene expression was compared and discussed. It seems likely; information from different reactions of this fungus against different insect cuticles can be helpful in producing specific pesticides.

Keywords

Article Title [Persian]

آنالیز بیان کمی ژنهای دای‌پپتیدیل پپتیداز و سیتوکروم p450 مونواکسیژناز در Beauveria bassiana طی رشد روی کوتیکول حشرات

Authors [Persian]

  • معصومه کردی
  • ناصر فرخی
دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی، تهران، ج. ا. ایران
Abstract [Persian]

Beauveria bassiana یک قارچ بیمارگر حشرات است، که به عنوان یک عامل کنترل بیولوژیک می باشد. Beauveria bassiana با تخریب و نفوذ در کوتیکول به انواع حشرات حمله می­کند. با آنالیز بیان ژن های دای­پپتیدیل­پپتیداز و سیتوکروم p450 مونواکسیژناز با اسفاده از روش پی سی آر در زمان واقعی (Real-Time PCR) اساس مولکولی عمل این قارچ مورد بررسی قرار گرفت. بوریا باسیانا در یک محیط کشت مصنوعی و محیط های عصاره کوتیکولی چهار گونه از راسته های متفاوت حشرات رشد داده شد، و بیان دو ژن معرفی شده در قارچ در محیط کشت‌های مختلف (حاوی کوتیکول گونه های مختلف حشرات) مورد بررسی قرار گرفت. نتایج نشان داد بیان ژن­دای­پپتیدیل­پپتیداز در محیط­های حاوی عصاره های کوتیکول حشرات در مقایسه با محیط کشت مصنوعی افزایش بیان نشان داد، در حالیکه سیتوکروم p450  مونواکسیژناز کاهش بیان نشان داد. در نهایت تاثیر انواع کوتیکول در القای بیان ژن مقایسه و مورد بحث قرار گرفت. به نظر می­رسد اطلاعات حاصل از پاسخ متفاوت این قارچ در برابر کوتیکول حشرات مختلف می تواند در تولید آفت‌کش­های اختصاصی کمک کننده باشد.

Keywords [Persian]

  • بوریا باسیانا
  • بیان ژن
  • پی سی آر در زمان واقعی
  • رونوشت

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Iran Agricultural Research
Volume 40, Issue 2
March 2022
Pages 25-32

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