Seed germination prediction of osmotic-stressed safflower (Carthamus tinctorius L.) at different temperatures using hydrotime analysis

Document Type : Research Paper

Authors

1 Department of Agroecology, College of Agriculture and Natural Resources of Darab, Shiraz University, Shiraz, I. R. Iran

2 Department of Crop and Horticultural Science Research, Agricultural Research, Education and Extension Organization (AREEO), Fars Agricultural and Natural Resources Research and Education Center, Shiraz, I. R. Iran

Abstract

The hydrotime analysis can predict the germination of plants at reduced water potential (ψ). The effects of reduced ψ on seed germination properties in three safflower cultivars including Padideh, Faraman and Isfahan cultivars at 10, 20 and 30 ºC were analyzed using the hydrotime model. Five ψ values (0, -0.3, -0.6, -0.9 and -1.2 MPa) prepared in polyethylene glycol 6000 (PEG) solutions were used in the experiment. The results indicated that all three tested cultivars had the highest germination rate at 30º C and better tolerance to osmotic stress at 20º C. At 20º C, Faraman cultivar had the lowest medium base water potential [ψb(50)]. The standard deviation of ψb (σψb) among cultivars and temperatures could be as high as 0.52 MPa for the Faraman cultivar at 20º. Three tested cultivars had statistically different hydrotime constant (θH) at 10º C. The lowest ψb(50) for achieving 50% germination at 10º C was seen in the Isfahan cultivar, and at either 20º C or 30º C was seen in Faraman and Padideh cultivars. The suppressing effect of drought stress on seed germination was greater in Padideh and Faraman cultivars than that in the Isfahan cultivar. Overall, applying of hydrotime model allowed identifying osmotic stress-tolerant cultivars such as Isfahan during germination; and which might be helpful to seed germination prediction and providing more accurate information for sowing time of safflower.
Abbreviations: ψ (water potential); PEG (polyethylene glycol); ψb(50) (medium base water potential); θH (hydrotime constant); σψb (standard deviation of ψb); MGT (mean germination time); FGP (final germination percentage).

Keywords

Article Title [Persian]

پیش‌بینی جوانه‌زنی بذر گلرنگ در شرایط تنش اسمزی و درجه حرارت‌های مختلف با استفاده از تحلیل هیدروتایم

Authors [Persian]

  • سید مجتبی موسوی 1
  • احسان بیژن زاده 1
  • زهرا زینتی 1
  • لیلا نظری 2
1 بخش اگرواکولوژی دانشکده کشاورزی و منابع طبیعی داراب، دانشگاه شیراز، شیراز، ج. ا. ایران
2 بخش تحقیقات زراعی و باغی، مرکز تحقیقات کشاورزی و منابع طبیعی فارس، سازمان تحقیقات آموزش و ترویج کشاورزی فارس، شیراز، ج. ا. ایران
Abstract [Persian]

- تحلیل هیدروتایم، قادر به پیش‌بینی روند جوانه‌زنی با کاهش پتانسیل آب (ψ) می‌باشد. اثرات کاهش پتانسیل آب روی جوانه‌زنی بذر در سه رقم گلرنگ شامل رقم های پدیده، فرامان و اصفهان در دمای 10، 20 و 30 درجه سانتی‌گراد از طریق مدل هیدروتایم واکاوی شد. در این آزمایش پنج پتانسیل آب (ψ) (0، -0/3، -0/6، 0/9، -1/2- مگاپاسکال) که در محلول‌های پلی‌اتیلن گلیکول  6000 (PEG) تهیه شدند استفاده شد. . نتایج نشان داد که هر سه رقم مورد آزمایش، دارای بیشترین سرعت جوانه‌زنی در دمای 30 درجه‌ی سانتی‌گراد و تحمل بهتر تنش خشکی در دمای 20 درجه  سانتی­گراد بودند. در دمای 20 درجه‌ی سانتی‌گراد، رقم فرامان دارای کم‌ترین پتانسیل آب‌پایه متوسط ψb(50)   بود. در میان ارقام و دما های مختلف، رقم فرامان دارای بیشترین انحراف معیار  ψb (σψb) به میزان 0/52 مگاپاسکال در دمای 20 درجه سانتیگراد ­بود. ثابت هیدروتایم (θH)  در دمای 10 درجه بین سه رقم مورد آزمایش به طور معنی­داری متفاوت بود. پایین‌ترینψb(50)  برای دست‌یابی به جوانه‌زنی 50 درصد در 10 درجه سانتی­گراد، مربوط به رقم اصفهان بود و در دمای 20 یا 30 درجه سانتی­گراد، پایین‌ترین مقدار مربوط به رقم فرامان و پدیده بود. اثر مهارکنندگی تنش خشکی روی جوانه‌زنی بذر در ارقام پدیده و فرامان بیشتر از رقم اصفهان بود. به‌طورکلی، کاربرد مدل هیدروتایم، امکان شناسایی ارقام متحمل به تنش اسمزی مانند رقم اصفهان را در طی جوانه‌زنی فراهم کرده و می‌تواند در پیش‌بینی جوانه‌زنی بذر و ارائه اطلاعات دقیق‌تر در خصوص زمان کاشت گلرنگ سودمند باشد.

Keywords [Persian]

  • گلرنگ
  • سرعت جوانه‌زنی
  • ثابت هیدروتایم
  • پتانسیل آب

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Iran Agricultural Research
Volume 40, Issue 1
April 2021
Pages 83-92

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