Segmented linear Model to characterize tolerance to tomato yellow leaf curl virus and tomato leaf curl virus in two tomato cultivars under greenhouse conditions

Document Type : Research Paper

Authors

1 Department of Plant Production and Genetics, College of Agriculture, Shiraz University, Shiraz, I. R. Iran

2 -Department of Plant Production and Genetics, College of Agriculture, Shiraz University, Shiraz, I. R. Iran 2-Department of Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, I. R. Iran

Abstract

To investigate the amount of yield losses caused by Iranian  isolates of tomato yellow leaf curl virus (TYLCV-[Ab]) and tomato leaf curl Karnataka virus (ToLCKV-IR) in Rio Grande (RG) and Grosse Lisse (GL) tomato cultivars, tomato seedlingswere separately agroinoculated with  an infectious clone of either virus and disease development was monitored over four time intervals. After the emergence of symptoms, disease severity (DS) was evaluated visually using an ordinal rating scale and the data was converted to a ratio scale. Losses caused by the viruses were estimated by measuring vegetative indices including wet and dry weight and height of the aerial and underground parts of the plants. Initial DS in plants infected by ToLCKV-IR was less than those infected by TYLCV-[Ab]. Descending order of rates of disease increase for the studied pathosystems was as follows: ToLCKV-IR-infected GL>TYLCV-Ab-infected GL> TYLCV-[Ab]-infected GL> ToLCKV-IR-infected RG. With delayed inoculation, DS was reduced in both cultivars inoculated with either of the two viruses. A segmented linear model showed a very good fit to the data of relative biomass and duration of exposure of all pathosystems. The estimated parameters of the model were used to evaluate different aspects of the damage curve. The highest crop loss disease threshold was observed for GL cultivar irrespective of the inoculated virus. Similar results were achieved when the desensitization disease level was evaluated. Curvilinear tolerance or slope did not show any significant difference between the infected-GL plants to either virus and also TOLCKV-IR-RG, while TYLCV-[Ab]-RG showed the highest curvilinear tolerance. Findings of this study can be applied in screening for tolerant tomato plants.

Keywords

Article Title [Persian]

مدل تجربی خطی تکه ای برای توصیف جنبه های مختلف تحمل به ویروس پیچیدگی برگ زرد گوجه فرنگی و ویروس پیچیدگی برگ گوجه فرنگی در دو رقم گوجه فرنگی در شرایط گلخانه ای

Authors [Persian]

  • حبیب اله حمزه زرقانی 1
  • سید علی اکبر بهجت نیا 2
  • ساره دلنواز 1
1 1گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ج. ا. ایران
2 1گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ج. ا. ایران 2ستاد مرکز تحقیقات ویروس شناسی گیاهی، بخش گیاهپزشکی، دانشکده
Abstract [Persian]

به منظور بررسی میزان خسارت محصول بوسیله جدایه‌های ایرانی ویروس پیچیدگی برگ زرد گوجه فرنگی (TYLCV-[Ab]) و ویروس کارناتاکای پیچیدگی برگ گوجه فرنگی (ToLCKV-IR) بر روی ارقام گوجه فرنگی ریوگراند (RG) و گروس لیزی (GL)، گیاهچه‌های گوجه فرنگی با همسانه‌های عفونت زای هر ویروس بطور جداگانه با روش آگرواینوکولیشن مایه زنی شدند و پیشرفت بیماری در چهار فاصله زمانی پایش گردید. پس از ظهور علائم شدت بیماری بصورت چشمی و با استفاده از یک مقیاس نمره دهی ترتیبی ارزیابی شد و داده ها به مقیاس نسبی تبدیل شدند. خسارات ویروس ها با اندازه گیری شاخص های رشد شامل وزن تر و خشک و ارتفاع بخش های هوائی و زیرزمینی گیاهان برآورد شد. شدت اولیه بیماری در گیاهان آلوده به ToLCKV-IR کمتر از گیاهان آلوده به TYLCV-[Ab] بود. حداکثر سرعت افزایش بیماری در گیاهان رقم GL آلوده به ToLCKV-IR مشاهده شد و بعد از آن به ترتیب سرعت افزایش بیماری گیاهان آلوده به TYLCV-[Ab] از هر دو رقم قرار گرفتند و کمترین سرعت افزایش بیماری در گیاهان GL آلوده به ToLCKV-IR مشاهده شد. با تأخیر در زمان مایه زنی، شدت بیماری در هر دو رقم آلوده به هر یک از دو ویروس کاهش یافت. در همه پاتوسیستم ها، مدل خطی تکه ای برازش خیلی خوبی به زیست توده نسبی و مدت تماس نشان داد. پارامترهای برآورد شده مدل برای ارزیابی جنبه های مختلف نمودار خسارت استفاده شد. صرفنظر از ویروس مایه زنی شده به گیاه بالاترین آستانه خسارت بیماری در رقم GL مشاهده شد. وقتی سطح حساسیت زدائی بیماری ارزیابی شد، نتایج مشابهی حاصل شد. تحمل منحنی- خطی یا شیب بین گیاهان GL آلوده به هر یک از دو ویروس و گیاهان RG آلوده به ToLCKV-IR تفاوت معنی داری نشان نداد، ولی رقم RG آلوده به TYLCV-[Ab] بالاترین تحمل منحنی- خطی را نشان داد. نتایج این پژوهش می تواند در غربال گری برای گیاهان گوجه فرنگی متحمل استفاده شود.

Keywords [Persian]

  • مدل خطی تکه ای
  • ویروس پیچیدگی برگ گوجه فرنگی
  • گوجه فرنگی
  • ویروس پیچیدگی برگ زرد گوجه فرنگی

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Iran Agricultural Research
Volume 39, Issue 2
December 2020
Pages 17-28

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