Evaluation of anesthetic potency of peppermint extract (Mentha piperita) and its physiological and histological effects on the Persian sturgeon (Acipenser persicus) juveniles

Document Type : Research Paper

Authors

Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I. R. Iran

Abstract

ABSTRACT- Anesthesia is a practical method for reducing stress in aquatic organisms. This study assessed the effectiveness of peppermint extract as an herbal anesthetic, along with its physiological and histopathological effects on Persian sturgeon (Acipenser persicus). Ninety fish were exposed to peppermint extract at concentrations of 0, 150, 250, 500, 750, and 1000 mg/L. To evaluate the effects of the solvent, an additional 75 fish were exposed to ethanol at concentrations of 450, 750, 1500, 2250, and 3000 mg/L. Ethanol exposure did not produce significant anesthesia, physiological changes, or tissue damage (P > 0.05). Peppermint extract successfully induced general anesthesia, particularly at concentrations between 300 and 500 mg/L, with both induction and recovery times averaging under five minutes. However, concentrations above 500 mg/L led to hematological changes and moderate tissue damage. Observed lesions included gill hyperplasia, congestion, epithelial lifting, liver degeneration and vacuolization, and necrosis in kidney tubules. Hematological analysis showed significant increases in hematocrit and hemoglobin levels, indicating possible stress or respiratory impairment, while red blood cell and white blood cell counts remained unchanged compared to the controls. A concentration of 150 mg/L was effective in reducing stress, while 300–500 mg/L is recommended for achieving optimal anesthesia.

Keywords

Main Subjects

Article Title [Persian]

ارزیابی قدرت بیهوشی عصاره روغنی نعناع فلفلی، عصاره نعناع فلفلی و اثرات فیزیولوژیکی و بافت شناسی آن بر بچه ماهیان خاویاری ایرانی، Acipenser persicus Borodin

Authors [Persian]

  • سارا والی
  • محمد مازندرانی
  • محمد سوداگر
  • محمدرضا ایمانپور
گروه آبزیان، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ج. ا. ایران
Abstract [Persian]

بیهوشی روشی مژثر برای کاهش استرس موجودات آبزی است. این مطالعه به بررسی اثربخشی عصاره نعناع فلفلی به عنوان یک بی حس کننده گیاهی و اثرات فیزیولوژیکی و هیستوپاتولوژیک آن بر ماهیان خاویاری ایرانی (Acipenser persicus) پرداخت. 90 ماهی در معرض عصاره نعناع فلفلی در غلظت‌های 0، 150، 250، 500، 750 و 1000 میلی‌گرم در لیتر قرار گرفتند، در حالی که 75 ماهی تحت تأثیر اتانول (450، 750، 1500، 2250 و 3000 میلی‌گرم در لیتر) قرار گرفتند. قرار گرفتن در معرض اتانول منجر به بیهوشی قابل توجهی، تغییرات فیزیولوژیکی یا آسیب بافتی نشد (P > 0.05). عصاره نعناع به طور موثر بیهوشی عمومی را، به ویژه در غلظت های 300-500 میلی گرم در لیتر، با زمان القاء و بهبودی به طور متوسط ​​کمتر از پنج دقیقه القا کرد. با این حال، غلظت بیش از 500 میلی گرم در لیتر منجر به تغییرات خونی و آسیب بافتی متوسط، از جمله هیپرپلازی، احتقان، و بلند کردن اپیتلیال در آبشش ها، و همچنین دژنراسیون و واکوئل شدن در کبد، و نکروز در لوله های کلیه شد. تجزیه و تحلیل هماتولوژیک افزایش قابل توجهی در سطوح هماتوکریت (Hct) و هموگلوبین (Hb) نشان داد که نشان دهنده استرس یا اختلال عملکرد تنفسی است، بدون تغییرات قابل توجهی در تعداد گلبول های قرمز (RBC) یا گلبول های سفید خون (WBC) در مقایسه با گروه شاهد. غلظت 150 میلی گرم در لیتر به طور مؤثر استرس را کاهش می دهد، در حالی که 300-500 میلی گرم در لیتر برای بیهوشی مطلوب توصیه می شود.

Keywords [Persian]

  • سمیت
  • خونشناسی بیهوشی
  • عصاره گیاهی
  • ماهی آب شیرین

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Iran Agricultural Research
Volume 44, Issue 1
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April 2025
Pages 131-142

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