Cellulase production by the fungus Trichoderma reesei was studied using sugar beet pulp (SBP) as a substrate. The subculture medium was a salt solution consisting of KH2PO4, CaCl2, etc. Fungal cells were sub-cultured in an orbital shaker (180 rpm) at 30°C for 1-2 generations (two days for each generation) and were then used as an inoculum. Exponential cells were inoculated into a medium containing SBP. Data showed that cellulose in SBP has a potential to be used as a substrate to produce cellulase. The effect of cellulose concentration on cellulase production was investigated. Using the medium composition (the mixture of chemical salts and SBP substrate) and a fermentation time of 4-6 days, a maximum cellulase activity of 0.46 IU/ml of filter paper activity was obtained.
Moosavi-Nasab, M. and Majdi-Nasab, M. (2008). Cellulase Production by Trichoderma reesei using Sugar Beet Pulp. Iran Agricultural Research, 25.26(1.2), 107-116. doi: 10.22099/iar.2008.190
MLA
Moosavi-Nasab, M. , and Majdi-Nasab, M. . "Cellulase Production by Trichoderma reesei using Sugar Beet Pulp", Iran Agricultural Research, 25.26, 1.2, 2008, 107-116. doi: 10.22099/iar.2008.190
HARVARD
Moosavi-Nasab, M., Majdi-Nasab, M. (2008). 'Cellulase Production by Trichoderma reesei using Sugar Beet Pulp', Iran Agricultural Research, 25.26(1.2), pp. 107-116. doi: 10.22099/iar.2008.190
CHICAGO
M. Moosavi-Nasab and M. Majdi-Nasab, "Cellulase Production by Trichoderma reesei using Sugar Beet Pulp," Iran Agricultural Research, 25.26 1.2 (2008): 107-116, doi: 10.22099/iar.2008.190
VANCOUVER
Moosavi-Nasab, M., Majdi-Nasab, M. Cellulase Production by Trichoderma reesei using Sugar Beet Pulp. Iran Agricultural Research, 2008; 25.26(1.2): 107-116. doi: 10.22099/iar.2008.190
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