اولین گزارش از وقوع ویروس وای سیب زمینی در برخی از گیاهان زینتی در ایران

نوع مقاله : مقاله کوتاه

نویسندگان

1 مرکز تحقیقات ویروس شناسی گیاهی، دانشکده کشاورزی شیراز، دانشگاه شیراز، شیراز، ج. ا، ایران

2 گروه کشاورزی، دانشگاه پیام نور، تهران، ج. ا، ایران

چکیده

طی بررسی‌‌‌های انجام شده از فضای سبز شهر شیراز در تابستان 1401، علائمی شامل موزاییک و کلروز روی برگ­های گیاهان زینتی کوکب­کوهی (Rudbeckia hirta)، یک گونه کوکب (Dahlia sp.) و پتونیای مکزیکی (Ruellia brittoniana) مشاهده شد. آر ان ای کل بطور جداگانه از برگ­های 10 نمونه­ی دارای علائم و یک نمونه بدون علائم بعنوان کنترل منفی استخراج و با استفاده از یک جفت آغازگر دژنره پوتی­ویروس­ها (Nib2F, Nib3R) در واکنش رونویسی معکوس- زنجیره­ای پلی­مزار (RT-PCR)  بکار رفت. RT-PCR منجر به تکثیر یک قطعه دی­ان­ای با اندازه مورد انتظار (حدود 350 جفت­باز) در تمام نمونه­های دارای علائم شد، در­حالی‌که هیچ قطعه‌ی دی ان ای از گیاه بدون علائم مورد آزمایش تکثیر نشد. ترادف نوکلئوتیدی  به­روش توالی­یابی سنگر اندازه قطعه دی­ان­ای تکثیرشده را دقیقاً 350 جفت­باز تعیین و  تأیید نمود که متعلق­ به ژن Nib ویروس وای سیب‌زمینی (PVY) است. مقایسه توالی نوکلئوتیدی آمپلیکون‌های جدایه­های مورد­ مطالعه با توالی نوکلئوتیدی همان ناحیه از ژنوم برخی دیگراز جدایه­های PVY موجود در بانک ژن نشان داد که جدایه PVY آلوده­کننده پتونیای­مکزیکی (R. brittoniana) بیشترین شباهت (8/98%) را با جدایه­ی PVY از آمریکا با رس‌شمارKY_848029.1  و جدایه­­های PVY کوکب­کوهی (R. hirta) و یک گونه کوکب (Dahlia sp.) بیشترین شباهت (بترتیب 3/98% و 4/98) را با یک جدایه­ PVY از قزاقستان با رس شمار ON_583980.1 دارند.

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