بررسی الگوی بیان برخی از ژن های ناقل نیتروژن در ریشه در دو رقم مختلف گندم

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه اصلاح نباتات و بیوتکنولوژی، دانشکده تولیدات گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ج. ا. ایران

چکیده

در تولید محصولات زراعی، یکی از عوامل محدود کننده به ویژه در دسترس بودن نیتروژن (N) و مشتقات N است، زیرا آنها در متابولیسم و رشد گیاه اهمیت زیادی دارند. ژن های مختلف ناقل نیترات جذب، انتقال و جابجایی مجدد نیتروژن را در گیاهان واسطه می کنند. با این حال، نقش آنها با توجه به مراحل پر شدن دانه و عملکرد کلی گیاه کاملاً درک نشده است. در اینجا، بیان تعدادی از ژن‌ها و آنزیم‌های دارای نقش در جذب و متابولیسم نیتروژن در طول دوره گرده‌افشانی و در شرایط تیمار N و کمبود N در دو ژنوتیپ گندم مروارید و گنبد اندازه‌گیری شد. نمونه‌ها 10، 15 و 20 روز پس از گرده‌افشانی که مرحله‌ای حیاتی در پر شدن دانه است، گرفته شد و ارتباط بین بیان ژن، فعالیت آنزیم و روزهای پس از گرده‌افشانی نشان داده شد. مشاهده شد که بیان ژن مربوط به N به طور قابل توجهی در شرایط کمبود N و در طول دوره گرده‌افشانی افزایش می‌یابد، که نشان‌دهنده اهمیت ژن‌ها و آنزیم مربوط به N در حفظ متابولیسم و رشد گیاهان در شرایط کمبود N است.

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