شناسایی باکتریهای شایع رورست و درون رست بذر برنج موثر در تقویت رشد و جوانه زنی گیاه برنج

نوع مقاله : مقاله کامل

نویسندگان

1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه بو علی سینا، همدان، ج. ا. ایران

2 گروه تحقیقات اصلاح و تهیه بذر، موسسه تحقیقات برنج ایران، سازمان تحقیقات، آموزش و ترویج کشاورزی، رشت، ج. ا. ایران

3 گروه گیاهپزشکی، موسسه تحقیقات برنج ایران، سازمان تحقیقات، آموزش و ترویج کشاورزی، رشت، ج. ا. ایران

چکیده

چکیده- در طول فصل زراعی، بذور برنج فاقد علائم آلودگی از شالیزارهای مختلف استان گیلان، ایران، جمع آوری شدند. از میان 39 جـدایه‌ی باکـتریایی شامل 19 اپی‌فیت و 20 اندوفیت، پنج گـروه تاکـسونومی بر اساس هـضم آنـزیمی مـحصول PCR ناحیه‌ی 16S r-DNA به دست آمدند. بر اساس آزمون‌های بیوشیمیایی و توالی‌یابی ناحیه‌ی16S r-DNAدر مجموع اندوفیت و اپی‌فیت بذور برنج، پرجمعیت‌ترین گروه‌های تاکسونومی، گروه‌های V و II ، به ترتیب به عنوان Pantoea ananatis و Pseudomonas oryzihabitans  شناسایی گردیدند. شش جدایه‌ی نماینده از این دو گروه جهت ارزیابی توانایی‌شان در تقویت رشد و جــوانه‌زنی بذر برنج انتخاب شدند. در بین آنها، P. oryzihabitans فــاقد اثر افزایــشی موثر بود ولی جدایه JpB1 باکتری P. ananatis   به عنوان موثرترین جدایه‌ی تقویت کننده‌ی رشد در نظر گرفته شد، زیرا این جدایه اثرات رشدی با ثباتی روی اکثر صفات مورد بررسی در هر دو آزمون جوانه‌زنی بذر و رشد برنج داشت. اگرچه جدایه OpB3 باکتری P. Ananatis میزان بیشتری اکسین تولید نمود و فسفات بیشتری نسبت به سایر جدایه‌ها حل نمود، اما در تقویت جوانه زنی بذر برنج اثرات رشدی موثری نداشت.

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