Detection of quinolones residues in beef and chicken meat in hypermarkets of Urmia, Iran using ELISA

Document Type: Full Article

Authors

1 Department of Food Hygiene; Karaj Branch; Islamic Azad University, Karaj; Karaj, I. R. Iran

2 Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, I. R. Iran

3 Graduated from Faculty of Veterinary Medicine , Islamic Azad University, Karaj Branch, Karaj, I. R. Iran

Abstract

ABSTRACT- The aim of this study was to determine the quinolone residues in beef and chicken meat samples collected from Urmia region local markets.A total of 395 beef and chicken meat samples varied in three various brands were analyzed in Urmia, Iran by using a sensitive and reliable analytic method based on ELISA detection, for quantification of quinolone antibiotics residues. Samples preparation was performed according to the instructions of the Ridascreen kit (RBiopharm AG, Darmstadt, Germany).Two hundred seventeen of 395 (54.9%) examined chicken meat and beef samples were found to be positive for quinolone antibiotic residue. Seventy-nine number (48.7%) of beef samples and 138 number (59.2%) of chicken meat samples were contaminated to quinolone residues. The mean levels of quinolone antibiotic residue were found to be 37.86± 0.57 μg/kg in positive chicken samples and the mean levels of quinolone residues were as low as 5.51± 1.17 μg/kg in positive beef samples. Present study indicated that 395 samples of beef and chicken meat sold in Urmia contained residues of quinolone antibiotics. In terms of preventing antibiotic resistance in humans, the low level of quinolone residue levels observed in this study represents a positive result for local food control.
 

Keywords


Article Title [Persian]

باقیمانده کینولون در گوشت مرغ و گوساله در فروشگاههای ارومیه، با استفاده از روش الایزا

Authors [Persian]

  • زهره مشاک 1
  • علی مجدر لنگرودی 2
  • تورج مهدی زاده 2
  • ایوب عبادی فتح آباد 2
  • امیر هومان اسدی 3
1 دانشگاه آزاد اسلامی، واحد کرج
2 گروه بهداشت و کنترل کیفی مواد غذائی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ج. ا. ایران.
3 فارغ التحصیل دکتری دامپزشکی، دانشگاه آزاد اسلامی، واحد کرج، کرج، ج. ا. ایران.
Abstract [Persian]

چکیده - هدف از این مطالعه بررسی باقیمانده­های کینولون در گوشت مرغ و گوساله که از سوپرمارکت­های شهر ارومیه نمونه­برداری گردیده شده می­باشد. در مجموع تعداد 395 نمونه از گوشت مرغ و گوشت گوساله در سه برند متفاوت به وسیله آزمون حساس و قابل اعتماد الایزا جهت ارزیابی میزان کینولون در شهر ارومیه مورد بررسی قرار گرفتند.دآماده­سازی نمونه­ها بر اساس روش ارائه شده در ریدااسکرین کیت انجام شد.
از مجموع 395 نمونه، 217 نمونه (54.9 %) از گوشت مرغ و گوشت گوساله از جهت وجود باقیمانده آنتی بیوتیکی مثبت ارزیابی گردید. 79 نمونه از گوشت گوساله (48.7 %) و 138 نمونه (59.2 %) از گوشت مرغ به باقیمانده آنتی بیوتیک کینولون آلوده بودند. میانگین باقیمانده آنتی بیوتیک کینولون در نمونه های مثبت گوشت مرغ 0.57± 37.86 و در نمونه­های مثبت گوشت گوساله 1.17 ± 5.51  میکروگرم/کیلوگرم بوده است. مطالعه حاضر نشان می­دهد که 395 نمونه خریداری شده گوشت مرغ و گوساله در شهر ارومیه حاوی باقیمانده آنتی بیوتیکی می­باشند. از جهت مقاومت آنتی بیوتیکی در انسان، میزان پایین باقیمانده آنتی بیوتیک در نمونه­های مطالعه حاضر نشاندهنده­ی شرایط مثبت کنترل مواد غذایی در این منطقه است.
 
 

Keywords [Persian]

  • کلمات کلیدی: کینولون
  • باقیمانده
  • گوساله
  • گوشت مرغ
  • الایزا
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