First report of the incidence of potato virus Y in some ornamental plants in Iran

Document Type : Note

Authors

1 Plant Virology Research Center, School of Agriculture, Shiraz University, Shiraz, I. R. Iran

2 Agriculture Department, Payame Noor University, Tehran, I. R. Iran

10.22099/iar.2023.48019.1550

Abstract

During the surveys conducted from green space in Shiraz City, Iran, in the summer of 2022, leaf chlorosis and mosaic symptoms were observed on the leaves of black-eyed-susans (Rudbeckia hirta), a  Dahlia sp., and Mexican Petunia (Ruellia brittoniana) plants. Total genomic RNA was separately extracted from 10 symptomatic and one symptomless (negative control) leaf samples and subjected to Reverse Transcription-Polymerase Chain Reaction (RT-PCR) using a potyvirus degenerate primer pair (NIb3R, NIb2F). RT-PCR resulted in the amplification of an approximately 350 bp DNA fragment in all symptomatic samples, while no such fragment was amplified from the symptomless plant. The amplified DNA fragment was subjected to Sanger sequencing, and its size was determined to be exactly 350 bp, and confirmed that it belongs to the Nib gene of potato virus Y (PVY). The nucleotide (nt) sequence of the amplicons was compared with the nt sequence of the same region of some other PVY isolates that were available in the GenBank. The sequence analysis revealed that the R. brittoniana isolate exhibited the highest (98.8%) similarity to a PVY isolate from the USA with the Acc. No. of KY_848029.1. Similarly, the R. hirta and the Dahlia isolates showed the highest (98.3%, 98.4 %, respectively ) similarity to a PVY isolate from Kazakhstan with the Acc. No. of ON_583980.1.

Keywords

Article Title [Persian]

اولین گزارش از وقوع ویروس وای سیب زمینی در برخی از گیاهان زینتی در ایران

Authors [Persian]

  • مهرداد صالح زاده 1
  • علیرضا افشاریفر 1
  • سعیده دهقانپور فراشاه 2
1 مرکز تحقیقات ویروس شناسی گیاهی، دانشکده کشاورزی شیراز، دانشگاه شیراز، شیراز، ج. ا، ایران
2 گروه کشاورزی، دانشگاه پیام نور، تهران، ج. ا، ایران
Abstract [Persian]

طی بررسی‌‌‌های انجام شده از فضای سبز شهر شیراز در تابستان 1401، علائمی شامل موزاییک و کلروز روی برگ­های گیاهان زینتی کوکب­کوهی (Rudbeckia hirta)، یک گونه کوکب (Dahlia sp.) و پتونیای مکزیکی (Ruellia brittoniana) مشاهده شد. آر ان ای کل بطور جداگانه از برگ­های 10 نمونه­ی دارای علائم و یک نمونه بدون علائم بعنوان کنترل منفی استخراج و با استفاده از یک جفت آغازگر دژنره پوتی­ویروس­ها (Nib2F, Nib3R) در واکنش رونویسی معکوس- زنجیره­ای پلی­مزار (RT-PCR)  بکار رفت. RT-PCR منجر به تکثیر یک قطعه دی­ان­ای با اندازه مورد انتظار (حدود 350 جفت­باز) در تمام نمونه­های دارای علائم شد، در­حالی‌که هیچ قطعه‌ی دی ان ای از گیاه بدون علائم مورد آزمایش تکثیر نشد. ترادف نوکلئوتیدی  به­روش توالی­یابی سنگر اندازه قطعه دی­ان­ای تکثیرشده را دقیقاً 350 جفت­باز تعیین و  تأیید نمود که متعلق­ به ژن Nib ویروس وای سیب‌زمینی (PVY) است. مقایسه توالی نوکلئوتیدی آمپلیکون‌های جدایه­های مورد­ مطالعه با توالی نوکلئوتیدی همان ناحیه از ژنوم برخی دیگراز جدایه­های PVY موجود در بانک ژن نشان داد که جدایه PVY آلوده­کننده پتونیای­مکزیکی (R. brittoniana) بیشترین شباهت (8/98%) را با جدایه­ی PVY از آمریکا با رس‌شمارKY_848029.1  و جدایه­­های PVY کوکب­کوهی (R. hirta) و یک گونه کوکب (Dahlia sp.) بیشترین شباهت (بترتیب 3/98% و 4/98) را با یک جدایه­ PVY از قزاقستان با رس شمار ON_583980.1 دارند.

Keywords [Persian]

  • آغازگر‌های عمومی پوتی‌ویروس
  • شیراز
  • فضای سبز
  • گیاهان زینتی

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Iran Agricultural Research
Volume 42, Issue 1
October 2023
Pages 47-54

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