Rise of activity of antioxidant enzymes and secondary metabolites under treatment with LED lights in two genotypes of Melissa officinalis

Document Type : Note

Authors

1 Department of Plant Science, Faculty of Science, Shahrekord University, Shahrekord, I. R. Iran

2 Department of Plant Science, Faculty of Science, Shahrekord University & Research Institute of Biotechnology, Shahrekord University, Shahrekord, I. R. Iran

3 Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, I. R. Iran

10.22099/iar.2023.48567.1560

Abstract

The innovation of light-emitting diodes (LEDs) has provided a new opportunity to grow plants in controlled environments. In this study, the plantlets of two genotypes of lemon balm were put for seven weeks in an incubator containing LED lamps with red, blue, red + blue (70:30 ratio), and white light spectrum with a light intensity of 300 μmol m-2s-1 and with a light cycle of 16 hours of light and 8 hours darkness and temperature of 25±2°C, and to compare with these conditions; some plantlets were also put in the greenhouse. The plantlets grown in incubators with red + blue LED lamps had higher activity of some antioxidant enzymes including catalase, guaiacol peroxidase, and ascorbate peroxidase, higher amounts of phenolic compounds and the valuable active substance of rosmarinic acid (the latter only in Ilam genotype), and phenylalanine ammonia lyase (PAL) enzyme activity than the plantlets grown in the greenhouse. However, in the Isfahan genotype significant difference in the content of rosmarinic acid was observed only between white LED light with other LED and greenhouse lights. Overall, according to the results of the current study, it can hope to use LED light sources to improve the growth and qualitative and quantitative properties of the medicinal plant, lemon balm, and also to create high-quality plants.

Keywords

Main Subjects

Article Title [Persian]

افزایش فعالیت آنزیم‌های آنتی اکسیدانی و متابولیت‌های ثانویه تحت تیمار با لامپ‌های LED در دو ژنوتیپ .Melissa officinalis L

Authors [Persian]

  • طیبه احمدی 1
  • لیلا شبانی 2
  • محمد رضا سبزعلیان 3
1 گروه علوم گیاهی، دانشکده علوم، دانشگاه شهرکرد، ج. ا. ایران
2 گروه علوم گیاهی، دانشکده علوم، دانشگاه شهرکرد، ج. ا. ایران و گروه علوم گیاهی، دانشکده علوم و پژوهشکده بیوتکنولوژی، دانشگاه شهرکرد، ج. ا. ایران
3 گروه تولید و‌ ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه صنعتی اصفهان، اصفهان، ج. ا. ایران
Abstract [Persian]

چکیده - نوآوری دیودهای ساطع‌کننده نور (LED) فرصت جدیدی را برای رشد گیاهان در محیط های کنترل شده فراهم کرده است. در مطالعه حاضر، گیاهچه-های دو ژنوتیپ بادرنجبویه به مدت 7 هفته در انکوباتور حاوی لامپ های LED قرمز، آبی، قرمز + آبی (نسبت 70:30) و سفید با شدت نور 300 میکرومول بر مترمربع بر ثانیه و با چرخه نور 16 ساعت روشنایی و 8 ساعت تاریکی و دمای 2±25 درجه سانتیگراد قرار داده شد و برای مقایسه با این شرایط، تعدادی گیاهچه از هر دو ژنوتیپ در گلخانه قرار داده شد. گیاهچه های قرار داده شده در انکوباتور با لامپ های LED قرمز + آبی دارای فعالیت بالاتر آنزیم‌های آنتی‌اکسیدان (کاتالاز، گایاکول پراکسیداز و آسکوربات پراکسیداز)، فعالیت بیشتر آنزیم فنیل آلانین آمونیالیاز(PAL) ، مقادیر بیشتر ترکیبات فنلی، و ماده موثره ارزشمند رزمارینیک اسید (در ژنوتیپ ایلام) نسبت به گیاهچه های کاشته شده در گلخانه بودند. هرچند در ژنوتیپ اصفهان تفاوت معنی‌دار در میزان رزمارینیک اسید فقط بین نور سفید LED با سایر نورهای LED و گلخانه‌ای مشاهده شد. در مجموع، با توجه به نتایج مطالعه حاضر می توان به استفاده از منابع نور LED برای بهبود رشد و خواص کمی و کیفی گیاه دارویی بادرنجبویه (Melissa officinalis)  و همچنین ایجاد گیاهان باکیفیت بالای این گونه امیدوار بود.

Keywords [Persian]

  • آنزیم‌های آنتی‌اکسیدی
  • بادرنجبویه
  • ترکیبات فنلی
  • کیفیت نور
  • نور LED

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Iran Agricultural Research
Volume 42, Issue 1
October 2023
Pages 41-46

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