Identification of predominant epiphytic and endophytic bacterial isolates in rice seeds effective for enhancement of seed germination and plant growth

Document Type: Full Article


1 Department of Plant Protection, College of Agriculture, Bu-Ali Sina University, Hamedan, I. R. Iran

2 Department of Agronomy and Plant Breeding, Rice Research Institute of Iran (RRII), Agricultural Research Education and Extension Organization (AREEO), Rasht, I. R. Iran

3 Department of Plant Pathology, Rice Research Institute of Iran (RRII), Agricultural Research Education and Extension Organization (AREEO), Rasht, I. R. Iran


ABSTRACT-During rice growing season, the symptomless rice seeds from different paddy fields in Guilan province, Iran, were collected. After isolation of epiphytic and endophytic bacteria, 39 isolates including 19 epiphytes and 20 endophytes were selected based on the predominant characteristics. Five Operational Taxonomic Units (OTUs) were obtained based on PCR-RFLP of 16S r-DNA in these isolates. According to biochemical tests and partial sequencing of 16S-rDNA, in both epiphyte and endophyte bacteria of rice seeds, the most populated OTUs (V and II) were identified as Pantoea ananatis and Pseudomonas oryzihabitans, respectively. Six representative isolates from these two OTUs were selected to be evaluated for their abilities for rice seed germination and growth enhancement. Among them, P. oryzihabitans was not beneficially effective. However, JpB1 isolate of P. ananatis was considered to be the most effective plant growth promoting isolate, since it showed stable beneficial effects on most surveyed characteristics in both rice seed germination and growth enhancement experiments.  Although, OpB3 isolate of P. ananatis produced IAA in higher amount and solubilized phosphate more than the other isolates followed by JpB1 and P. ananatis L3pB3, it was not beneficially effective on rice seed germination.


Article Title [Persian]

شناسایی باکتریهای شایع رورست و درون رست بذر برنج موثر در تقویت رشد و جوانه زنی گیاه برنج

Authors [Persian]

  • ریحانه غلامعلی زاده 1
  • غلام خداکرمیان 1
  • علی ‏اکبر عبادی 2
  • مریم خشکدامن 3
1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه بو علی سینا، همدان، ج. ا. ایران
2 گروه تحقیقات اصلاح و تهیه بذر، موسسه تحقیقات برنج ایران، سازمان تحقیقات، آموزش و ترویج کشاورزی، رشت، ج. ا. ایران
3 گروه گیاهپزشکی، موسسه تحقیقات برنج ایران، سازمان تحقیقات، آموزش و ترویج کشاورزی، رشت، ج. ا. ایران
Abstract [Persian]

چکیده- در طول فصل زراعی، بذور برنج فاقد علائم آلودگی از شالیزارهای مختلف استان گیلان، ایران، جمع آوری شدند. از میان 39 جـدایه‌ی باکـتریایی شامل 19 اپی‌فیت و 20 اندوفیت، پنج گـروه تاکـسونومی بر اساس هـضم آنـزیمی مـحصول PCR ناحیه‌ی 16S r-DNA به دست آمدند. بر اساس آزمون‌های بیوشیمیایی و توالی‌یابی ناحیه‌ی16S r-DNAدر مجموع اندوفیت و اپی‌فیت بذور برنج، پرجمعیت‌ترین گروه‌های تاکسونومی، گروه‌های V و II ، به ترتیب به عنوان Pantoea ananatis و Pseudomonas oryzihabitans  شناسایی گردیدند. شش جدایه‌ی نماینده از این دو گروه جهت ارزیابی توانایی‌شان در تقویت رشد و جــوانه‌زنی بذر برنج انتخاب شدند. در بین آنها، P. oryzihabitans فــاقد اثر افزایــشی موثر بود ولی جدایه JpB1 باکتری P. ananatis   به عنوان موثرترین جدایه‌ی تقویت کننده‌ی رشد در نظر گرفته شد، زیرا این جدایه اثرات رشدی با ثباتی روی اکثر صفات مورد بررسی در هر دو آزمون جوانه‌زنی بذر و رشد برنج داشت. اگرچه جدایه OpB3 باکتری P. Ananatis میزان بیشتری اکسین تولید نمود و فسفات بیشتری نسبت به سایر جدایه‌ها حل نمود، اما در تقویت جوانه زنی بذر برنج اثرات رشدی موثری نداشت.

Keywords [Persian]

  • باکتری‌های مفید
  • کودهای زیستی
  • تقویت رشد
  • تولید هورمون گیاهی
  • باکتری‌های همراه بذر برنج
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