Positive effects of karrikin on seed germination of three medicinal herbs under drought stress

Document Type: Full Article

Authors

1 Department of Agronomy, College of Agriculture, University of Zabol, Zabol, I. R. Iran

2 Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, I. R. Iran

Abstract

ABSTRACT- Improper seed germination is a serious challenge for medicinal herbs under drought stress conditions in semi-arid and arid regions. A new group of plant growth regulators known as karrikins have been proved useful to improve seed germination in some plants. In this regard, the effects of karrikin on seed germination and vigor of three medicinals under drought stress were examined. Seeds of Trachyspermum copticum (L.) Link, Foeniculum vulgare Miller, and Cuminum cyminum L. were sowed under drought treatments (-1, -1.5, -2 and -2.5 MPa of PEG 6000), with and without synthetic Karrikin GR24 (10µM). Results indicated increasing osmotic pressure seed germination characteristics were severely reduced; however, karrikin significantly increased the seed germination of the three herbs. The application of karrikin in all drought conditions significantly increased germination percentage, germination rate, germination index, seedling vigor, shoot length and radicle length of all herb seedlings. Ajwain (T. copticum) showed a greater drought tolerance compared to the other medicinal species.

Keywords


Article Title [Persian]

تأثیر مثبت کاریکین بر جوانه زنی بذر سه گیاه دارویی تحت تنش خشکی

Authors [Persian]

  • محسن موسوی نیک 1
  • ابوالفضل جوکار 2
  • عبدالرحمان رحیمیان بوگار 2
1 دانشگاه زابل
2 بخش علوم باغبانی، دانشکده کشاورزی، دانشگاه شیراز
Abstract [Persian]

چکیده- جوانه زنی نامناسب بذر گیاهان دارویی در مناطق نیمه خشک و خشک یک چالش مهم به حساب می آید. مشخص شده است که یک گروه جدید از تنظیم کننده های رشد گیاهی، معروف به کاریکین ها، برای بهبود جوانه زنی بذر برخی گیاهان مفید هستند. در این راستا تأثیر کاریکین بر جوانه زنی و قدرت دانهال سه گیاه دارویی تحت تنش خشکی مورد بررسی قرار گرفت. بذرهای زنیان (Trachyspermum copticum (L.) Link)، رازیانه (Foeniculum vulgare Miller) و زیره سبز (Cuminum cyminum L.)  تحت تیمارهای خشکی (1-، 5/1-، 2- و 5/2-  MPa  از (PEG 6000 با یا بدون استفاده از کاریکین سنتزی GR24 (10µM) کشت شدند. اگرچه با افزایش فشار اسمزی مشخصات جوانه زنی بذر به شدت کاهش یافت، اما کاریکین بطور معنی داری فاکتورهای جوانه زنی سه گیاه دارویی را افزایش داد. کاربرد کاریکین در تمام شرایط خشکی بطور  معنی داری درصد جوانه زنی، سرعت جوانه زنی، شاخص جوانه زنی، قدرت دانهال، طول شاخساره و طول ریشه چه تمام دانهال های گیاهان دارویی را افزایش داد. زنیان (T. copticum) در مقایسه با دیگر گونه های دارویی تحمل به خشکی بیشتری نشان داد.

Keywords [Persian]

  • واژه های کلیدی:
  • استریگولاکتون
  • قدرت دانهال
  • Cuminum cyminum Foeniculum vulgare Trachyspermum
Agrawal, R. (2002). Seed technology. Pub. Co. LTD. New Delhi. India.

Alam, M.M., Nahar, K., & Hasanuzzaman, M. (2014). Exogenous jasmonic acid modulates the physiology, antioxidant defense and glyoxalase systems in imparting drought stress tolerance in different Brassica species. Plant Biotechnology Reports, 8, 279-293.

Akhavan Armaki, M., Azarnivand, H., Assareh, M.H., Jaafari, A.A., & Tavili, A. (2013). Evaluation of drought stress effects on germination indices of four genotypes of rangeland species. Bromus tomentellus, 6692, 167-177. (In Persian)

Bahmani, K., IzadiDarbandi, A., & SadatNoori, S.A. (2013). Evaluation of the contents and essential oil constituents of some ecotypes of Iranian fennel. Journal of Crops Improvement, 15(4), 13-24. (In Persian)

Behnamnia, M., Kalantari, K.M., & Rezanejad, F. (2009). Exogenous application of brassinosteroid alleviates drought-induced oxidative stress in Lycopersicon esculentum L. General and Applied Plant Physiology, 35, 22-34.

Cheng, X., Ruyter-Spira, C., & Bouwmeester, H. (2013). The interaction between strigolactones and other plant hormones in the regulation of plant development. Frontiers in Plant Science, 4, 1-16.

Chiwocha, S.D.S., Dixon, K.W., Flematti, G.R., Ghisalberti, E.L., Merritt,  D.J., Nelson, D.C., Riseborough, J.M., Smith, S.M., & Stevens, J.C. (2009). Karrikins: A new family of plant growth regulators in smoke. Plant Science, 177, 252-256.

Coons, J., Coutant, N., Lawrence, B., Finn, D., & Finn, S. (2014). An effective system to produce smoke solutions from dried plant tissue for seed germination studies. Applications in Plant Science, 2, 1-3. 

Dixon, K.W., Merritt, D.J., Flematti, G.R., & Ghisalberti, E.L. (2009). Karrikinolide - a phytoreactive compound derived from smoke with applications in horticulture, ecological restoration and agriculture. Acta Horticulturae, 813, 155-170.

Ghebrehiwot, H.M., Kulkarini, M.G., Szalai, G., Soòs, V., Balázs, E., & VanStaden, J. (2013). Karrikinolide residues in grassland soils following fire: Implications on germination activity. South African Journal of Botany, 88, 419-424.

Giri, J. (2011). Glycinebetaine and abiotic stress tolerance in plants. Plant Signaling & Behavior, 6, 1746-1751.

Gomes, M.M.A., Netto, A.T., Campostrini, E., Bressan Smith, R., Zullo, M.A.T., Ferraz, T.M., Siqueira, L.N., Leal, N.R., & NúñezVázquez, M. (2013). Brassinosteroid analogue affects the senescence in two papaya genotypes submitted to drought stress. Theoretical and Experimental Plant Physiology, 25, 186-195.

Guterres, J., Rossato, L., Pudmenzky, A., Doley, D., Whittaker, M., & Schmidt, S. (2013). Micron-size metal-binding hydrogel particles improve germination and radicle elongation of Australian metallophyte grasses in mine waste rock and tailings. Journal of Hazardous Materials, 248, 442-450.

Ha, C.V., Leyva González, M.A., Osakabe, Y., Tran, U.T., Nishiyama, R., Watanabe, Y., Tanaka, M., Seki, M., Yamaguchi, S., Dong, N.V., Yamaguchi Shinozaki, K., Shinozaki, K., Herrera Estrella, L., & Tran, L.S. (2014). Positive regulatory role of strigolactone in plant responses to drought and salt stress. Proceedings of the National Academy of Sciences of the USA, 111(2), 851-856.

Habibi, G. (2012). Exogenous salicylic acid alleviates oxidative damage of barley plants under drought stress. Acta Biologica Szegediensis, 56, 57-63.

Halford, B. (2010). Smoke Signals. Chemical & Engineering News, 88, 37-38.

Hedge, I.C., & Lamond, J.M. (1987). Trachyspermum. Flora Iranica, 162, 336-338.

Hussain, S., Ali, A., Ibrahim, M., Saleem, M.F., Haji, M.A., & Bukhsh, A. (2012). Exogenous application of abscisic acid for drought tolerance in sunflower (Helianthus annuus L.): A review. The Journal of Animal & Plant Sciences, 22, 806-826.

International Seed Testing Association. (2008). International rules for seed testing. Seed Science and Technology, 13, 356-513.

Jakab, G., Ton, J., Flors, V., Zimmerli, L., Me´traux, J.P., & MauchMani, B. (2005). Enhancing arabidopsis salt and drought stress tolerance by chemical priming for its abscisic acid responses. Plant Physiology, 139, 267-274.

Kang, G.Z., Li, G.Z., Liu, G.Q., Xu, W., Peng, X.Q., Wang, C.Y., Zhu, Y.J., & Guo, T.C. (2013). Exogenous salicylic acid enhances wheat drought tolerance by influence on the expression of genes related to ascorbate-glutathione cycle. Biologia Plantarum, 57, 718-724.

Kaya, M.D., Okçu, G., Atak, M., Çikili, Y., & Kolsarici, Ö. (2006). Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 23, 291-295.

Kissoudis, C., Van de Wiel, C., Visser, R.G.F., & Van der Linden, G. (2014). Enhancing crop resilience to combined abiotic and biotic stress through the dissection of physiological and molecular crosstalk. Frontiers in Plant Science, 5, 1-20.

Li, J., Yin, L.Y., Jongsma, M.A., & Wang, C.Y. (2011). Effects of light, hydropriming and abiotic stress on seed germination, and shoot and root growth of pyrethrum (Tanacetum cinerariifolium). Industrial Crops & Products, 34, 1542-1549.

Li, Y., Zhaoa, H., Duana, B., Korpelainen, H., & Li, C. (2011). Effect of drought and ABA on growth, photosynthesis and antioxidant system of Cotinus coggygria seedlings under two different light conditions. Environmental and Experimental Botany, 71, 107-113.

Long, R.L., Stevens, J.C., Griffiths, E.M., Adamek, M., Powles, S.B., & Merritt, D.J. (2011). Detecting karrikinolide responses in seeds of the Poaceae. Australian Journal of Botany, 59, 610-620.

Maguire, J.D. (1962). Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2, 176-177.

Mos, M., Binek, A., Zielinski, A., & Wojtowicz, T. (2007). Effect of osmotic stress on vigor in naked and husked oat cultivars subjected to accelerated ageing. American-Eurasian Journal of Agricultural and Environmental Science, 5, 465-469.

Nair, J.J., Munro, O.Q., Pošta, M., Papenfus, H.B., Beier, P., & VanStaden, J. (2012). X-ray crystallographic structure determination of the smoke-derived karrikin KAR3. South African Journal of Botany, 88, 107-109.

Nazarli, H., Ahmadi, A., & Hadian, J. (2014). Salicylic acid and methyl jasmonate enhance drought tolerance in chamomile plants. Journal of HerbMed Pharmacology, 3, 87-92.

Niazi, M.H., & Raja, M.R. (1971). Effect of NPK on the yield of white zeera (Cuminum Cyminum L.). Journal of Agricultural Research, 9(2), 124-127.

Rohamare, Y., Dhumal, K.N., & Nikam, T.D. (2014). Response of ajowan to water stress induced by polyethylene glycol (PEG) 6000 during seed germination and seedling growth. Journal of Environmental Biology, 35, 789-793.

RahimianBoogar, A., Salehi, H., & Jowkar, A. (2014). Exogenous nitric oxide alleviates oxidative damage in turfgrasses under drought stress. South African Journal of Botany, 92, 78-82.

Rao, S.R., Qayyum, A., Razzaq, A., Ahmad, M., Mahmood, I., & Sher, A. (2012). Role of foliar application of salicylic acid and L-tryptophan in drought tolerance of maize. The Journal of Animal & Plant Sciences, 22, 768-772.

Rauf, M., Munir, M., UIHassan, M., Ahmed, M., & Afzai, M. (2007). Performance of wheat genotypes under osmotic stress at germination and early seedling growth stage. African Journal of Biotechnology, 6, 971-275.

RivasArancibia, S.P., Montaña, C., VelascoHernández, J.X., & Zavala Hurtado, J.A. (2006). Germination responses of annual plants to substrate type, rainfall, and temperature in a semi-arid inter-tropical region in Mexico. Journal of Arid Environments, 67, 416-427.

Sun, Y.Y., Sun, Y.J., Wang, M.T., Li, X.Y., Guo, X., Hu, R., & Ma, J. (2010). Effects of seed priming on germination and seedling growth under water stress in rice. Acta Agronomica Sinica, 36, 1931-1940.

Sunmonu, T.O., Kulkarni, M.G., & VanStaden, J. (2016). Smoke-water, karrikinolide and gibberellic acid stimulate growth in bean and maize seedlings by efficient starch mobilization and suppression of oxidative stress. South African Journal of Botany, 102, 4-11.

Tekrony, D.M., & Egli, D.B. (1991). Relationship of seed vigor to crop yield: a review. Crop science, 21, 816-822.

Ton, J., Jakab, G., Toquin, V., Flors, V., Iavicoli, A., Maeder, M.N., Metraux, J.P., & MauchMani, B. (2005). Dissecting the β-aminobutyric acid induced priming phenomenon in Arabidopsis. Plant Cell, 17, 987-999.

VanDenBerg, L., & Zeng, Y.J. (2006). Response of South African indigenous grass species to drought stress induced by polyethylene glycol (PEG) 6000. South African Journal of Botany, 72, 284-286.

Waters, M.T., Scaffidi, A., Flematti, G.R., & Smith, S.M. (2012). The origins and mechanisms of karrikin signalling. Current Opinion in Plant Biology, 16, 667-672.

Yamada, Y., Furusawa, S., Nagasaka, S., Shimomura, K., Yamaguchi, S., & Umehara, M. (2014). Strigolactone signaling regulates rice leaf senescence in response to a phosphate deficiency. Planta, 240, 399-408.

Zhang, L., Gao, M., Zhang, L., Li, B., Han, M., Alva, A.K., & Ashraf, M. (2013). Role of exogenous glycinebetaine and humic acid in mitigating drought stress-induced adverse effects in Malus robusta seedlings. Turkish Journal of Botany, 37, 920-929.

Zeng, Y.J., Wang, Y.R., & Zhang, J.M. (2010). Is reduced seed germination due to water limitation a special survival strategy used by xerophytes in arid dunes? Journal of Arid Environments, 74, 508-511.