Determination of standardized prececal protein digestibility of canola meal in British United Turkeys Big 6 at different ages using multiple linear regression procedure


Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, I. R. Iran


ABSTRACT- Ninety six 2-week-old British United Turkeys (BUT) Big 6 were assigned to 2 groups (4 replicates of 12 birds each). One basal diet and another one in which 20% canola meal replaced the corn starch in the basal diet were prepared. At 9, 16 and 21 wk of age, four birds from each pen slaughtered for digesta collection. The last two-third segment of the intestine, between the Meckel’s diverticulum and about 2 cm anterior to the ileo-ceca-colonic junction, was excised. The diets and digesta were analyzed for dry matter, crude protein, and chromic oxide content. The standardized prececal protein digestibility of canola meal was calculated using multiple linear regression procedure. The standardized prececal protein digestibility of canola meal at 21 wk of age was significantly lower as compared with that of 9 wk of age; the relative weight of digestive organs also decreased significantly with increasing age (P< 0.05). By using multiple linear regression which accounted for the endogenous nitrogen losses, we showed that standardized prececal protein digestibility was also influenced by the age.


Main Subjects

Article Title [Persian]

تعیین گوارش پذیری استاندارد پیش سکومی پروتئین کنجاله کلزا

Authors [Persian]

  • محمد رضوانی
  • امیر اخلاقی
  • فاطمه صائمی
  • محمد مهدی امتی
  • محمد دادپسند
  • هادی آتشی
گروه علوم دامی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ج. ا. ایران.
Abstract [Persian]

چکیده- نود و شش قطعه بوقلمون نژاد انگلیسی بیگ-6 در سن 2 هفتگی به دو گروه (هر گروه شامل4 تکرار و هر تکرار 12 پرنده) تقسیم شدند. یک جیره ی پایه و یک جیره ی دارای 20 درصد کنجاله کانولا که جانشین نشاسته ی ذرت در جیره ی پایه شده بود، آماده شدند. در سن 9، 16 و 21 هفتگی چهار پرنده از هر تکرار برای جمع آوری مواد هضمی روده کشتار شدند. قطعه‏ی دو سوم انتهایی روده حدفاصل زایده مکل و 2 سانتی متر قبل از محل اتصال ایلئوم به سکوم بریده شد. ماده ی خشک، پروتئین خام و مارکر خارجی اکسید کروم در جیره های غذایی و مواد هضمی اندازه گیری شدند. گوارش پذیری استاندارد پیش سکومی پروتئین کنجاله کانولا با روش رگرسیون چند متغییره محاسبه شد. گوارش پذیری استاندارد پیش سکومی پروتئین کنجاله کانولا در سن 21 هفتگی به طور معنی داری کمتر از سن 9 هفتگی بود و وزن نسبی اندامهای گوارشی نیز روند کاهشی معنی داری را با افزایش سن نشان داد  (P< 0.05). با وجود تصحیح انجام شده برای نیتروژن دارای منشاء داخلی در روش رگرسیون خطی چند متغییره، مشاهده شد که گوارش پذیری استاندارد پیش سکومی پروتئین کنجاله کانولا تحت تاثیر سن پرنده قرار دارد.

Keywords [Persian]

  • واژه های کلیدی:
  • گوارش پذیری استاندارد پروتئین
  • رگرسیون چند متغیره
  • کنجاله کانولا
  • بوقلمون های نژاد انگلیسی بیگ 6
Aguilera, J.F., Prieto, E., Molina, E., & Lachica, M. (1988). A micromethod for routine determination of chromic dioxide in nutrition studies. Analusis, 16, 454-457.

Adedokun, S.A., Parsons, C.M., Lilburn, M.S., Adeola, O., & Applegate, T.J. (2007a). Comparison of ileal endogenous amino acid flows in broiler chicks and turkey pullets. Poultry Science, 86, 1682-1689.

Adedokun, S.A., Parsons, C.M., Lilburn, M.S., Adeola, O., & Applegate, T.J. (2007b). Standardised ileal amino acid digestibility of meat and bone meal from different sources in broiler chicks and turkey pullets with a nitrogen-free or casein diet. Poultry Science, 86, 2598-2607.

AOAC (1990). Official Methods of Analysis. Association of Official Analytical Chemists. 15th edition. Washington, DC.

Donkoh, A., & Moughan, P.J. (1999). Endogenous ileal nitrogen and amino acid flows in the growing pig receiving a protein-free diet and diets containing enzymatically hydrolyzed casein or graded levels of meat and bone meal. Animal Science, 68, 511-518.

Huang, K.H., Ravindran, V., Li, X., & Bryden, W.L. (2005). Influence of age on the apparent ileal amino acid digestibility of feed ingredients for broiler chickens. British Poultry Science, 46, 236-245.

Jansman, A.J.M., Smink, W., VanLeeuwen, P., & Rademacher, M. (2002). Evaluation through literature data of the amount and amino acid composition of basal endogenous crude protein at the terminal ileum of pigs. Animal Feed Science and Technology, 98, 49-60.

Kadim, T.I., Moughan, P.J., & Ravindran, V. (2002). Ileal amino acid digestibility assay for the growing meat chicken: Comparison of ileal and excreta amino acid digestibility in the chicken. British Poultry Science, 44, 588-597.

Kluth, H., Mantel, M., Elwert, C., & Rodehutscord, M. (2005). Variation in prececal amino acid and energy digestibility between pea (Pisum sativum) cultivars determined using a linear regression approach. British Poultry Science,46, 325-332.

Lemme, A., Ravindran, V., & Bryden, W.L. (2004). Ileal digestibility of amino acid in feed ingredients for broilers. World’s Poultry Science Journal, 60, 423-437.

NRC (1994). Nutrient Requirements of Poultry. 9th rev. ed. National Research Council, National Academy Press, Washington, DC.

Nordheim, J.P., & Coon, C.N. (1984). A comparison of four methods for determining available lysine in animal protein meal. Poultry Science, 63, 1040-1051.

Payne, W.L., Kifer, R.R., Snider, D.G., & Combs, G.F. (1971). Studies of protein digestion in chicken. 1. Investigation of apparent amino acid digestibility of fish meal protein using cecectomised, adult male chickens. Poultry Science, 50: 143-150.

Perez, L., FernandezFigarez, I., Nieto, R., Aguilera, F., & Prieto, C. (1993). Amino acid ileal digestibility of some grain legume seeds in growing chickens. Animal Production, 56, 261-267.

Ravindran, V., Hew, L.I., Ravindran, G., & Bryden, W.L. (2004). Endogenous amino acid flow in the ileum: Quantification using three techniques. British Journal of Nutrition, 92, 217-223.

Rezvani, M., Kluth, H., Woitow, G., & Rodehutscord, M. (2007). Studies on the effect of age and caecectomy on amino acid excretion and digestibility in laying hens. European Poultry Science, 71, 241-246.

Rezvani, M., Kluth, H., & Rodehutscord, M. (2008a). Comparison of amino acid digestibility determined prececally or based on total excretion of cecectomized laying hens. Poultry Science, 87, 2311-2319.

Rezvani, M., Kluth, H., Elwert, C., & Rodehutscord, M. (2008b). Effect of ileum segment and protein sources on net disappearance of crude protein and amino acids in laying hens. British Poultry Science, 49, 28-36.

Rezvani, M., Kluth, H., Bulang, M., & Rodehutscord, M. (2012). Variation in amino acid digestibility of rapeseed meal studied in caecectomised laying hens and relationship with chemical constituents. British Poultry Science, 53: 665-674.

Rodehutscord, M., Kapocius, M., Timmler, R., & Dieckmann, A. (2004). Linear regression approach to study amino acid digestibility in broiler chickens. British Poultry Science, 45, 85-92.

SAS (2004). SAS User’s Guide. Statistics. Version 9.2. SAS Institute Inc., Cary, NC.

Sauer, W.C., Fan, M.Z., Mosenthin, R., & Drochner, W. (2000). Methods for measuring ileal amino acid digestibility in pigs, in: D’Mello, JPF (Ed.) Farm Animal Metabolism and Nutrition, pp. 279-306, CAB International, Wallingford, UK.

Short, F.J., Wiseman, J., & Boorman, K.N. (1999). Application of a method to determine ileal digestibility in broilers of amino acids in wheat. Animal Feed Science and Technology, 79, 195-209.

Siriwan, P., Bryden, W.L., Mollah, Y., & Annison, E.F. (1993). Measurement of endogenous amino acid losses in poultry. British Poultry Science,  34, 939-949.

Sibbald, I.R. (1986). The TME system of feed evaluation: methodology, feed composition data and bibliography. Technical Bulletin 1986-4E. Animal Research Center, Research Branch, Agriculture Canada, Ottawa, Canada.

Sibbald, I.R. (1987). Estimation of bioavailable amino acids in feedstuffs for poultry and pigs: A review with emphasis on balance experiments. Canadian Journal of Animal Science, 67, 221-300.

Tarvid, I. (1995). The development of protein digestion in poultry. Poultry and Avian Biology Reviews, 6, 35-54.

Williams, C.H. David, D.J., & Iismaa, O. (1962). The determination of chromic oxide in feces samples by atomic absorption spectrophotometry. Journal of Agricultural Science, 59, 381-385.

Zuprizal, M.L., & Chagnea, A.M. (1992). Effect of age and sex on true digestibility of amino acids of rapeseed and soybean meals in growing broilers. Poultry Science, 71, 1486-1492.