Role of Ni-resistant rhizobacteria in the growth and Ni-uptake of maize in a calcareous soil

Document Type: Full Article


1 Department of Soil Science, Faculty of Agriculture, Tabriz. University, Tabriz, I. R. Iran

2 Department of Soil Science Engineering, Faculty of Agricultural Engineering and Technology, Tehran University, Tehran, I. R. Iran

3 Department of Soil Science Engineering,. College of Agriculture, Shiraz University, Shiraz, I. R. Iran


ABSTRACT- A pot experiment was conducted to elucidate the effects of single and co-inoculation of maize plant with Bacillus mycoides and Micrococcus roseus, indigenous to HMs contaminated soils, on the plant growth and uptake of essential nutrients and Ni by maize in the soil polluted by 250 and 500 mg Ni kg-1. At each level of Ni contamination, shoot dry weight and nutrients uptake significantly increased in M. roseus and B. mycoides treatments compared to non-inoculated plants. The highest plant growth promoting effect was found for B. mycoides at the level of 250 mg Ni kg-1 and for M. roseus at the level of 500 mg Ni kg-1 which enhanced plant biomass by 33.2% and 90%, respectively, compared to non-inoculated plants. At the levels of 250 and 500 mg Ni kg-1, shoot Ni uptake of plants inoculated with B. mycoides or M. roseus significantly increased compared to non-inoculated plants. Root Ni uptake of plants inoculated with B. mycoides and M. roseus significantly decreased at the level of 250 mg Ni kg-1 and increased at the level of 500 mg Ni kg-1 compared to control plants. The lowest Ni transfer factor and maximum Ni translocation factor were in plants inoculated with B. mycoides at the level of 250 mg Ni kg-1 and in non-inoculated plants at the levels of 500 mg Ni kg-1. Inoculation of plants with B. mycoides and M. roseus may be the effective treatments in Ni phytoextraction at the levels of 250 and 500 mg Ni kg-1, respectively. Consortium of two bacteria had the lowest plant dry matter and shoots and roots Ni uptake and the maximum transfer factor compared to other treatments at the level of 500 mg Ni kg-1 that may be the effective treatment in Ni phytostabilization.


Main Subjects

Article Title [Persian]

نقش ریزوباکتریهای مقاوم به نیکل در رشد و جذب نیکل گیاه ذرت در یک خاک آهکی

Authors [Persian]

  • الهام ملک زاده 1
  • حسینعلی علیخانی 2
  • غلامرضا ثواقبی فیروآبادی 2
  • مهدی زارعی 3
1 گروه علوم و مهندسی خاک، دانشکده کشاورزی،، دانشگاه تبریز، تبریز، ج. ا. ایران.
2 گروه مهندسی علوم خاک، دانشکده مهندسی و فناوری کشاورزی، دانشگاه تهران، تهران، ج. ا. ایران
3 گروه مهندسی علوم خاک، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ج. ا. ایران.
Abstract [Persian]

چکیده- اثر مایه‏زنی جداگانه و توام دو جدایه Bacillus mycoides و Micrococcus roseus بومی خاک‏های آلوده به فلزات سنگین بر رشد گیاه و جذب عناصر غذایی ضروری و نیکل توسط گیاه ذرت در خاک آلوده به سطوح 250 و 500 میلی‏گرم نیکل  در کیلوگرم خاک طی آزمایش گلدانی بررسی گردید. در هر یک از سطوح نیکل، وزن خشک اندام‏هوایی و جذب عناصر غذایی در تیمارهای M. roseus و B. mycoides در مقایسه با شاهد افزایش معنی‏داری داشتند. بیشترین اثر محرک رشد گیاه در سطح 250 میلی‏گرم نیکل در کیلوگرم در تیمار B. mycoides و در سطح 500 میلی‏گرم نیکل در کیلوگرم در تیمار M. roseus مشاهده گردید که به ترتیب نسبت به گیاهان شاهد مایه‏زنی نشده افزایش 2/33 و 90 درصدی داشتند. در سطوح 250 و 500 میلی‏گرم نیکل در کیلوگرم، جذب نیکل در اندام هوایی گیاهان مایه‏زنی شده با B. mycoidesو M. roseusبه‏طور معنی‏داری در مقایسه با گیاهان مایه‏زنی نشده افزایش داشت. جذب نیکل در ریشه گیاهان مایه‏زنی شده با B. mycoides و M. roseus به‏طور معنی‏داری در سطوح 250 و 500 میلی‏گرم نیکل در کیلوگرم در مقایسه با گیاهان شاهد به ترتیب کاهش و افزایش یافت. کمترین فاکتور انتقال از خاک به ریشه و بیشترین فاکتور انتقال از ریشه به اندام‏هوایی در سطح 250 میلی‏گرم نیکل در کیلوگرم در گیاهان مایه‏زنی شده با B. mycoides و در سطح 500 میلی‏گرم نیکل در کیلوگرم در گیاهان مایه‏زنی نشده بود. احتمالا مایه‏زنی گیاهان با  B. mycoidesو M. roseusبه ترتیب در سطوح 250 و 500 میلی‏گرم نیکل در کیلوگرم تیمارهای موثری در استخراج گیاهی نیکل باشند. در سطح 500 میلی‏گرم نیکل در کیلوگرم، تیمار مایه‏زنی مشترک با دو باکتری کمترین وزن خشک گیاهی و جذب نیکل به اندام‏هوایی و ریشه، و بیشترین فاکتور انتقال از خاک به ریشه را در مقایسه با دیگر تیمارها داشت و ممکن است تیمار موثری در تثبیت گیاهی نیکل باشد.

Keywords [Persian]

  • واژه های کلیدی:
  • باکتری‏های ریزوسفری مقاوم به نیکل
  • جذب عناصرغذایی
  • ذرت
  • نیکل
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