The effects of arbuscular mycorrhizal fungus and water stress on some antioxidant enzymes activities and nutrients uptake of two citrus rootstocks

Document Type: Full Article


Department of Soil Science, College of Agriculture, Shiraz University, Shiraz, I. R. Iran


ABSTRACT-Water stress is the main cause for crop yield reduction in the majority of agricultural regions of the world because it affects almost all plant functions. The effects of Glomus mosseae on growth, nutrients uptake, and antioxidant enzymes of sour orange (Citrus aurantium) and rough lemon) Citrus jambheri) rootstocks were assessed in sterilized soil under greenhouse conditions.  A three-factor experiment was set up in a completely randomized design with three replicates of each treatment. Treatments consisted of water stress at four levels (irrigation intervals of 2, 4, 6, and 8 days) and mycorrhizal treatments at two levels (inoculation with G. mosseae and non-mycorrhizal control). Mycorrhizal seedlings of two citrus rootstocks were successfully infected by G. mosseae. As water stress increased, root colonization, shoot dry weight, shoot N, P, Mn, Cu, and Fe uptake of two citrus rootstocks significantly decreased but shoot Zn uptake and the antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (G-POD) and ascorbate peroxidase (APX)] activities of two citrus rootstocks leaves increased. With inoculation of seedlings by G. mosseae compared with control, shoot dry weight, N and P uptake, and antioxidant enzymes activities increased. It may be concluded that mycorrhizal inoculation notably influenced shoot nutrients uptake and leaves antioxidant enzymes activities in citrus and an increase in these parameters alleviated water stress.


Main Subjects

Article Title [Persian]

اثر قارچهای میکوریز آربوسکولار و تنش آبی بر فعالیت برخی آنزیمهای آنتی اکسیدان و جذب عناصر غذای دو پایه مرکبات

Authors [Persian]

  • مهدی زارعی
  • زهرا پیمانه
  • عبدالمجید رونقی
بخش علوم خاک، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ج. ا. ایران.
Abstract [Persian]

چکیده- تنش خشکی از مهمترین فاکتورهای کاهش تولید در کشاورزی است زیرا بر تمام فعالیت‌های گیاه اثر دارد. در این پژوهش گلخانه‌ای اثر تنش خشکی و قارچ گلوموس موسه بر روی رشد، جذب عناصر غذایی و انزیم‌های آنتی اکسیدان دو پایه مرکبات ‌نارنج و رافلمون) در خاک استریل بررسی شد. در این مطالعه از سه فاکتور در قالب طرح کاملاً تصادفی با سه تکرار استفاده شد. فاکتور‌ها شامل سه سطح تنش خشکی (‌دور های آبیاری 2، 4، 6 و 8 روز)، میکوریز در 2 سطح ( تلقیح قارچ گلوموس موسه و شاهد) و دوپایه مرکبات ( نارنج و رافلمون) بودند. دانهال‌های میکوریزی دو پایه مرکبات به خوبی توسط قارچ گلوموس موسه کلنیزه شده بودند. با افزایش سطح تنش خشکی درصد کلنیزاسیون ریشه، عملکرد ماده خشک اندام هوایی، جذب نیتروژن، فسفر، منگنز، مس و آهن در اندام هوایی دو پایه مرکبات به طور معنی‌داری کاهش ولی جذب روی اندام هوایی و فعالیت آنزیم‌های آنتی اکسیدان ‌سوپراکسید دسموتاز، کاتالاز، گلوتاتیون پراکسیداز و آسکوربیک پراکسیداز) در برگ در هر دو پایه مرکبات افزایش یافت. با تلقیح دو پایه مرکبات با قارچ در مقایسه با گیاهان بدون قارچ عملکرد ماده خشک اندام هوایی، جذب نیتروژن، فسفر و آنزیم‌های آنتی اکسیدان افزایش یافت. ممکن است قارچ های میکوریز آربوسکولار با افزایش جذب عناصر غذایی  در اندام هوایی و فعالیت آنزیم‌های آنتی اکسیدان برگ  مرکبات اثرات تنش خشکی را تعدیل نماید.

Keywords [Persian]

  • واژه های کلیدی:
  • مرکبات
  • جذب عناصر غذایی
  • آنزیم‌های آنتی اکسیدان
  • تنش آبی
  • گلوموس موسه
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