Quantitative detection of chicken meat routine mislabeling in emulsion type sausages and burgers by SYBR green real time PCR assay

Document Type : Full Article

Authors

1 Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, I. R. Iran

2 Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, I. R. Iran

Abstract

ABSTRACT- Today, the authenticity of meat products with less costly and desirable species has increased. Therefore and considering religious, economicalor public health concerns, proper actions should be taken to prevent such frauds. In this study, real time PCR assay was applied for rapid, sensitive and specific identification and quantification of chicken tissue in meat products. Specific primers were designed on the 16SrRNA chicken mitochondrial genes. The conventional PCR and SYBR Green RT-PCR were employed on DNA extracted from 150 samples, sausages and burgers. The results showed the presence of misused chicken meat in the sausages and burgers were 84% and 26%, respectively. Additionally, the Q-PCR assay was able to detect estimate rates of 10% to 50% of chicken meat in the products. The detection limit of the method could be quantitatively used by regulation authorities to control the quality of meat products.

Keywords

Article Title [Persian]

شناسایی و اندازه‌گیری مقادیر غیر مجاز خمیر مرغ در محصولات گوشتی شامل سوسیس و برگر با استفاده از روش سایبرگرین

Authors [Persian]

  • لیلا لک‌زاده 1
  • سید شهرام شکرفروش 1
  • مهدی فاضلی 2
1 دانشگاه شیراز
2 دانشگاه شیراز
Abstract [Persian]

چکیده- امروزه توجه به تولید محصولات گوشتی بدون تقلب و با هزینه کمتر افزایش یافته است. بنابراین با در نظر گرفتن نگرانی‌های مربوط به سلامت عمومی اعتقادات مذهبی و مسائل اقتصادی، لازم است اقدامات مناسب جهت ممانعت از این تقلبات به عمل آید. در مطالعه حاضر با استفاده از آزمایش Real Time PCR به عنوان یک روش سریع، حساس و اختصاصی، به شناسایی و اندازه‌گیری میزان بافت‌های مرغی در محصولات گوشتی با برچسب تهیه شده از گوشت قرمز پرداخته شده است. بدین منظور، بر اساس ژن 16 rRNA ، پرایمرهای اختصاصی آماده گردید و با روشهای PCR و RT-PCR بر روی تعداد 150 DNA استخراج شده از نمونه‌های سوسیس و برگر انجام گردید. نتایج حاصل از مطالعه حاضر نشان دهنده وجود 84% و 26% بافت مرغ به ترتیب در نمونه‌های سوسیس و برگر می‌باشد. به علاوه روش RT-PCR(Q-PCR) قادر به تشخیص تقریبی 5-10% بافت مرغ در این محصولات است. این محدوده تشخیص توسط روش فوق می‌تواند به طور موثری توسط مراجع ذی‌صلاح به منظور کنترل کیفیت محصولات گوشتی مورد استفاده قرار گیرد.

Keywords [Persian]

  • واژه های کلیدی:
  • مرغ
  • سایبرگرینRT-PCR
  • 16S rRNA
  • برگر
  • سوسیس

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