Utilization of date syrup as a substrate for carotenoid production by Rhodotorula glutinis

Document Type: Research Paper


Department of Food Science and Technology, College of Agriculture, Shiraz University, Shiraz, I. R. Iran


The potential use of date syrup, for the production of carotenoids by Rhodotorula glutinisin batch fermentation process, was investigated during 7 days. The results revealed that carbon (glucose or date syrup) and nitrogen sources [yeast extract, (NH4)2SO4 and NH4NO3] had a significant influence on biomass and carotenoid production. Maximum yield of total carotenoid production (7.94 mg/L) with carotene content (2040 μg/g) and biomass (3.90 g/L) was obtained from R. glutinis after 7 days of fermentation in a substrate containing date syrup and yeast extract. The highest biomass (8.03 g/L) was obtained in the culture containing glucose and yeast extract, while the total carotenoid content of 6.72 mg/L with 836.86 μg/g carotene was produced in this medium. Significant differences were observed when comparing the average biomass and total carotenoid productions in different cultures and fermentation times. Our results demonstrated that date syrup (from low quality dates), as aby-product at a lower price, could be profitably used as a suitable carbon source for carotenoid production by R. glutinis.


Main Subjects

Article Title [Persian]

کاربرد شیره خرما به عنوان سوبسترا جهت تولید کاروتنوئید توسط رودوترولا گلوتینیس

Authors [Persian]

  • مرضیه موسوی نسب
  • الهه عابدی
  • سحر السادات موسوی نسب
دانشیار بخش علوم و صنایع غذایی، دانشگاه شیراز
Abstract [Persian]

امکان استفاده از شیره خرما جهت تولید کاروتنوئید توسط رودوترولا گلوتینیس، در فرایند تخمیر غیر مداوم طی 7 روز بررسی شد. نتایج نشان داد که منبع کربن (گلوکز یا شیره خرما) و منبع نیتروژن (عصاره مخمر، سولفات آمونیوم و نیترات آمونیوم) تاثیر قابل توجهی بر تولید زیست توده و کاروتنوئید داشت. حداکثر بازدهی تولید کاروتنوئید کل ( mg/L94/7) با محتوی کاروتنμg/g 2040 و زیست توده g/L 90/3 توسط رودوترولا گلوتنیس در مدت زمان 7 روزدر سوبسترای حاوی شیره خرما به عنوان تنها منبع کربوهیدرات و عصاره مخمر حاصل شد بیشترین میزان زیست توده g/L03/8 در محیط حاوی گلوکز و عصاره مخمر ایجاد شد، درحالی‌که میزان کاروتنوئید کل (mg/L 72/6) حاوی μg/g 86/836 کاروتن در این محیط تولید گردید. بین زمان‌های مختلف تخمیر و نیز محیط‌های کشت مختلف از نظر تولید زیست توده و تولید کاروتنوئید تفاوت معناداری مشاهده شد. نتایج حاصل از این پژوهش نشان داد که شیره خرما (از خرماهای با کیفیت پایین) به عنوان محصول جانبی با قیمت پایین می‌تواند به عنوان منبع کربن مناسب برای تولید کاروتنوئید توسط رودوترولا گلوتینیس مورد استفاده قرار گیرد.

Keywords [Persian]

  • محصول جانبی
  • شیره خرما
  • کاروتنوئید میکروبی
  • رودوترولا گلوتینیس
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