Utilization of date syrup as a substrate for carotenoid production by Rhodotorula glutinis

Document Type: Research Paper

Authors

Department of Food Science and Technology, College of Agriculture, Shiraz University, Shiraz, I. R. Iran

Abstract

The potential use of date syrup, for the production of carotenoids by Rhodotorula glutinisin batch fermentation process, was investigated during 7 days. The results revealed that carbon (glucose or date syrup) and nitrogen sources [yeast extract, (NH4)2SO4 and NH4NO3] had a significant influence on biomass and carotenoid production. Maximum yield of total carotenoid production (7.94 mg/L) with carotene content (2040 μg/g) and biomass (3.90 g/L) was obtained from R. glutinis after 7 days of fermentation in a substrate containing date syrup and yeast extract. The highest biomass (8.03 g/L) was obtained in the culture containing glucose and yeast extract, while the total carotenoid content of 6.72 mg/L with 836.86 μg/g carotene was produced in this medium. Significant differences were observed when comparing the average biomass and total carotenoid productions in different cultures and fermentation times. Our results demonstrated that date syrup (from low quality dates), as aby-product at a lower price, could be profitably used as a suitable carbon source for carotenoid production by R. glutinis.

Keywords

Main Subjects


Article Title [Persian]

کاربرد شیره خرما به عنوان سوبسترا جهت تولید کاروتنوئید توسط رودوترولا گلوتینیس

Authors [Persian]

  • مرضیه موسوی نسب
  • الهه عابدی
  • سحر السادات موسوی نسب
دانشیار بخش علوم و صنایع غذایی، دانشگاه شیراز
Abstract [Persian]

امکان استفاده از شیره خرما جهت تولید کاروتنوئید توسط رودوترولا گلوتینیس، در فرایند تخمیر غیر مداوم طی 7 روز بررسی شد. نتایج نشان داد که منبع کربن (گلوکز یا شیره خرما) و منبع نیتروژن (عصاره مخمر، سولفات آمونیوم و نیترات آمونیوم) تاثیر قابل توجهی بر تولید زیست توده و کاروتنوئید داشت. حداکثر بازدهی تولید کاروتنوئید کل ( mg/L94/7) با محتوی کاروتنμg/g 2040 و زیست توده g/L 90/3 توسط رودوترولا گلوتنیس در مدت زمان 7 روزدر سوبسترای حاوی شیره خرما به عنوان تنها منبع کربوهیدرات و عصاره مخمر حاصل شد بیشترین میزان زیست توده g/L03/8 در محیط حاوی گلوکز و عصاره مخمر ایجاد شد، درحالی‌که میزان کاروتنوئید کل (mg/L 72/6) حاوی μg/g 86/836 کاروتن در این محیط تولید گردید. بین زمان‌های مختلف تخمیر و نیز محیط‌های کشت مختلف از نظر تولید زیست توده و تولید کاروتنوئید تفاوت معناداری مشاهده شد. نتایج حاصل از این پژوهش نشان داد که شیره خرما (از خرماهای با کیفیت پایین) به عنوان محصول جانبی با قیمت پایین می‌تواند به عنوان منبع کربن مناسب برای تولید کاروتنوئید توسط رودوترولا گلوتینیس مورد استفاده قرار گیرد.

Keywords [Persian]

  • محصول جانبی
  • شیره خرما
  • کاروتنوئید میکروبی
  • رودوترولا گلوتینیس
Aksu, Z., & Eren, A.T. (2007). Production of carotenoids by the isolated yeast of Rhodotorula glutinis. Biochemical Engineering, 35, 107-113.

Al-Farsi, M., Alasalvar, C., Morris, A., Baron, M., & Shahidi, F. (2005a).Comparison of antioxidant activity, anthocyanins, carotenoids, and phenolics of three native fresh and sun-dried date (Phoenix dactylifera L.) varieties grown in Oman. Journal of agricultural and food chemistry American Chemical Society, 53, 7592-7599.

Al-Farsi, M., Alasalvar, C., Morris, A., Baron, M., & Shahidi F. (2005b). Compositional and sensory characteristics of three native sun-dried date (Phoenix dactylifera L.) varieties grown in Oman. Journal of agricultural and food chemistry American Chemical Society, 53, 7586-7591.

Al-Shahib, W., & Marshall, R.J. (2003). The fruit of the date palm Phoenix dactylifera L. Possible use as the best food of the future. Inter. Journal of Food Science, 54, 247-259.

AOAC, Official Methods of Analysis Association of Official Analytical Chemistry (2002). 17th (ed).The Association of Official Analytical Chemistry, In, Washington, DC.

Bhosale, P., &Gadre, R.V. (2001).Production of β-Carotene by a mutant of Rhodotorula glutinis. Applied Microbiology and Biotechnology, 55, 423-427.

Biglari, F., AlKarkhi, A.F.M., & Easa, A.M. (2008). Antioxidant activity and phenolic content of various date palm (Phoenix dactylifera) fruits from Iran. Journal of agricultural and food chemistry American Chemical Society, 107, 1636-1641.

Buzzini, P. (2000). An optimization study of carotenoid production by Rhodotorula glutinis DBVPG 3853 from substrates containing concentrated rectified grape must as the sole carbohydrate source. Journal of Industrial Microbiology and Biotechnology, 24, 41-45.

production was affected by nitrogen source. The best total carotenoids production rate could be obtained from culture contained date syrup and yeast extract as carbon and nitrogen sources, respectively.

 Buzzini, P. (2001). Batch and fed-batch carotenoid production by Rhodotorula glutinis Debaryomycescastellii co-cultures in corn syrup. Journal of Applied Microbiology, 90, 843-847.

Buzzini, P., & Martini A. (1999). Production of carotenoids by strains of Rhodotorula glutinis cultured in raw materials of agro-industrial origin. Bioresource Technology, 71, 41-44.

Córdova-Murueta1, J.H., García-Carreño1, F.L., de los Ángeles, M.,& del-Toro1, N.(2013). pH-Solubilzation Process as an Alternative to Enzymatic Hydrolysis Applied to Shrimp Waste. Turkish Journal of Fisheries and Aquatic Sciences, 13, 639-646.

Edge, R., McGravy, D.J., & Truscott, T.G. (1997). The carotenoids as antioxidants-are view. Journal of Photochemistry and Photobiology, 41, 189-200.

Frengova, G.I., Simova, E.D.,  Pavlova, K., Beshkova, D., & Grigorova, D. (1994). Formation of carotenoids by Rhodotorula glutinis in whey ultrafiltrate. Journal of Advanced Biotechnology and Bioengineering, 44, 888-894.

Hennekens, C.H. (1997). β-carotene supplementation and cancer prevention. Nutrition, 13, 697-699.

Munson, L.S., & Walker, P.H. (1906).The unification of reducing sugar methods. Journal of the American Oil Chemists' Society, 28, 663.

Mehaia, M.A., & Cheryan, M. (1991). Fermentation date extracts to ethanol and vinegar in batch and continuous membrane reactors. Enzyme and Microbial Technology, 13, 257-261.

Perrier, V., Dubreucq, E., & Glazy, P. (1995).Fatty acid and carotenoid composition of Rhodotorula strains. Archives of Microbiology, 164, 173-179.

Peterson, W.J., Evans, W.R., Leecce, E., Bell, T.A., & Etchells, J.L. (1958). Quantitative determination of the carotenoids in yeast of the genus Rhodotorula. Journal of Bacteriology, 75, 586-591.

Shih, C.T., Hang, & Y.D. (1996).Production of carotenoids by Rhodotorula rubra from sauerkraut brine. Le besm Wiss und-Technology, 29, 570-572.

Simpson, K.L., Chichester, C.O., & Phaff, H.J. (1971). Carotenoid pigments of yeast. In The yeasts, Vol. 2, A.H. Rose, J.S. Harrison (eds.), Academic Press, New York, pp. 493-515.