Protective effect of exogenous nitric oxide on alleviation of oxidative damage induced by high salinity in rice (Oryza sativa L.) seedlings

Document Type: Research Paper


1 Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, I. R. Iran

2 Department of Horticultural Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, I. R. Iran


To find the protective role of exogenous nitric oxide (NO) on salinity-stressed rice seedlings, a CRD-based factorial experiment with three replications was conducted in Agronomy Laboratory of the Faculty of Agricultural Sciences, University of Guilan, in 2012. The experimental design consisted of healthy and vigorous seedlings of two rice cultivars, Khazar and Goohar, the last already known as promising SA13 line, which were exposed to 0 (Control), 50 mM NaCl, 50 μM sodium nitroprusside (SNP) as NO donor solution supplemented with simultaneous 50 mM NaCl + 50 μM SNP for four days. After 4 days, electrolyte leakage and malondialdehyde (MDA) content, activities of antioxidant enzyme, destruction of chlorophyll and soluble protein content in leaves were measured in treated and control plants. The results showed that simultaneous treatment of rice leaves with SNP, suppressed the ion leakage content by 8.5% compared with the results of NaCl treatment. Furthermore, SNP increased the activities of superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX) and catalase (CAT). Exogenous application of NO also reduced peroxidation of membrane lipids, whereas increased the soluble protein content and chlorophyll pigments in rice leaves under salinity stress. These results suggested that NO could effectively protect rice seedlings from salt stress damaged by enhancing activities of antioxidant enzymes to quench the excessive reactive oxygen species caused by salt stress.


Main Subjects

Article Title [Persian]

نقش حفاظتی نیتریک‌اکساید برون‌زاد در کاهش خسارت اکسیداتیو القا‌شده با شوری شدید در دانهال‌های برنج

Authors [Persian]

  • سمانه اسدی صنم 1
  • محسن زواره 1
  • ابوذر هاشم پور 2
1 دانشگاه گیلان
2 دانشگاه گیلان
Abstract [Persian]

هدف این مطالعه، بررسی نقش حفاظتی نیتریک‌اکساید (NO) برون‌زاد در دانهال‌های برنج تحت تنش شوری بود. دانهال‌های قوی و سالم دو رقم برنج خزر و گوهر که پیشتر به‌عنوان لاین امید‌بخش SA13 شناخته شده بود، با محلول 50 میلی‌مولار نمک کلرید‌سدیم (NaCl)، 50 میکرومولار محلول سدیم نیتروپروساید (SNP) و کاربرد هم‌زمان 50 میلی‌مولار نمک کلریدسدیم و 50 میکرومولار محلول SNP همراه با شاهد (عدم کاربرد محلول) برای چهار روز تیمار شدند. پس از چهار روز، مقدار نشت یونی و مالون‌دی‌آلدهید (MDA)، فعالیت آنزیم‌های آنتی‌اکسیدانی، تخریب کلروفیل و مقدار پروتئین‌ محلول در برگ‌های گیاهان تیمار‌شده و شاهد اندازه‌گیری شد. نتایج نشان داد که تیمار هم‌زمان برگ‌های برنج با SNP موجب کاهش مقدار نشت یونی در حدود 5/8 درصد در مقایسه با کاربرد نمک کلریدسدیم شد. علاوه بر ‌این، SNP سبب افزایش فعالیت آنزیم‌های آنتی‌اکسیدانی سوپر‌اکسید‌ دیسموتاز (SOD)، پراکسیداز (POD)، آسکوربات پراکسیداز (APX) و کاتالاز (CAT) شد. هم‌چنین، کاربرد NO برون‌زاد موجب کاهش پراکسیده‌شدن لیپیدهای غشا، تأخیر در تجزیه پروتئین‌ها و تخریب رنگدانه‌های کلروفیل در برگ‌های برنج شد. این نتایج پیشنهاد می‌کند که NO می‌تواند دانهال‌های برنج را به‌طور مؤثری از خسارت ایجاد‌شده به‌وسیله تنش شوری با افزایش فعالیت آنزیم‌های آنتی‌اکسیدان در حذف گونه‌های فعال اکسیژن اضافی ناشی از تنش، محافظت کند.

Keywords [Persian]

  • آنزیم‌های آنتی‌اکسیدان
  • برنج
  • سدیم‌ نیتروپروساید
  • گونه‌های فعال اکسیژن
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